Sökresultat - "CRISPR"

Robust prediction of synthetic gRNA activity and cryptic DNA repair by disentangling cellular CRISPR cleavage outcomes.

Riesenberg S; Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany. stephan_riesenberg@eva.mpg.de., Kanis P; Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany., Karlic R; Bioinformatics Group, Division of Molecular Biology, Department of Biology, University of Zagreb, Zagreb, Croatia., Maricic T; Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany.

Journal Article , 2025

Assessment of Miniature AsCas12f1 Variants for Gene Editing and Activation.

Ren C; Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, Zhejiang, China.; ZJU-Hangzhou Global Scientific and Technological Innovation Center, Zhejiang University, Hangzhou, Zhejiang, China.; Institute of Bioengineering, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, Zhejiang, China., Bao Z; Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, Zhejiang, China.; ZJU-Hangzhou Global Scientific and Technological Innovation Center, Zhejiang University, Hangzhou, Zhejiang, China.; Institute of Bioengineering, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, Zhejiang, China.; Zhejiang Key Laboratory of Smart Biomaterials, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, Zhejiang, China.

Journal Article , 2025

Precision multiplexed base editing in human cells using Cas12a-derived base editors.

Schweitzer AY; Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT, USA.; Systems Biology Institute, Yale University, West Haven, CT, USA., Adams EW; Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT, USA.; Systems Biology Institute, Yale University, West Haven, CT, USA., Nguyen MTA; Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT, USA.; Systems Biology Institute, Yale University, West Haven, CT, USA., Lek M; Department of Genetics, Yale School of Medicine, New Haven, CT, USA., Isaacs FJ; Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT, USA. farren.isaacs@yale.edu.; Systems Biology Institute, Yale University, West Haven, CT, USA. farren.isaacs@yale.edu.; Department of Biomedical Engineering, Yale University, New Haven, CT, USA. farren.isaacs@yale.edu.

Journal Article , 2025

Array Assembler Provides Greatly Simplified crRNA Array Design for CRISPR Cas12 and Cas13 Variants.

Bryson JW; Biotech Research and Innovation Centre (BRIC), University of Copenhagen, Copenhagen 2200, Denmark.

Journal Article; Research Support, Non-U.S. Gov't , 2025

Post-cleavage target residence determines asymmetry in non-homologous end joining of Cas12a-induced DNA double strand breaks.

Chen RD; Key Laboratory of Laparoscopic Technology of Zhejiang Province, Department of General Surgery, Sir Run-Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310016, People's Republic of China.; Hangzhou Qiantang Hospital, Hangzhou, Zhejiang, 310018, People's Republic of China.; Institute of Translational Medicine, Zhejiang University School of Medicine, and Zhejiang University Cancer Center, Hangzhou, Zhejiang, 310029, People's Republic of China., Yang Y; Key Laboratory of Laparoscopic Technology of Zhejiang Province, Department of General Surgery, Sir Run-Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310016, People's Republic of China.; Institute of Translational Medicine, Zhejiang University School of Medicine, and Zhejiang University Cancer Center, Hangzhou, Zhejiang, 310029, People's Republic of China., Liu KM; Key Laboratory of Laparoscopic Technology of Zhejiang Province, Department of General Surgery, Sir Run-Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310016, People's Republic of China.; Institute of Translational Medicine, Zhejiang University School of Medicine, and Zhejiang University Cancer Center, Hangzhou, Zhejiang, 310029, People's Republic of China., Hu JZ; Key Laboratory of Laparoscopic Technology of Zhejiang Province, Department of General Surgery, Sir Run-Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310016, People's Republic of China.; Institute of Translational Medicine, Zhejiang University School of Medicine, and Zhejiang University Cancer Center, Hangzhou, Zhejiang, 310029, People's Republic of China., Feng YL; Key Laboratory of Laparoscopic Technology of Zhejiang Province, Department of General Surgery, Sir Run-Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310016, People's Republic of China.; Hangzhou Qiantang Hospital, Hangzhou, Zhejiang, 310018, People's Republic of China.; Institute of Translational Medicine, Zhejiang University School of Medicine, and Zhejiang University Cancer Center, Hangzhou, Zhejiang, 310029, People's Republic of China., Yang CY; Key Laboratory of Laparoscopic Technology of Zhejiang Province, Department of General Surgery, Sir Run-Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310016, People's Republic of China.; Institute of Translational Medicine, Zhejiang University School of Medicine, and Zhejiang University Cancer Center, Hangzhou, Zhejiang, 310029, People's Republic of China., Jiang RR; Institute of Translational Medicine, Zhejiang University School of Medicine, and Zhejiang University Cancer Center, Hangzhou, Zhejiang, 310029, People's Republic of China.; Zhejiang Key Laboratory of Multiomics and Molecular Enzymology, Yangtze Delta Region Institute of Tsinghua University, Jiaxing, Zhejiang, 314006, People's Republic of China., Liu SC; Key Laboratory of Laparoscopic Technology of Zhejiang Province, Department of General Surgery, Sir Run-Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310016, People's Republic of China.; Hangzhou Qiantang Hospital, Hangzhou, Zhejiang, 310018, People's Republic of China., Wang Y; Key Laboratory of Laparoscopic Technology of Zhejiang Province, Department of General Surgery, Sir Run-Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310016, People's Republic of China.; Institute of Translational Medicine, Zhejiang University School of Medicine, and Zhejiang University Cancer Center, Hangzhou, Zhejiang, 310029, People's Republic of China., Han PA; Institute of Animal Husbandry and Inner Mongolia Key Laboratory of Sugarbeet Genetics and Germplasm Enhancement, Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot, Inner Mongolia, 010031, People's Republic of China., Tian RG; Institute of Animal Husbandry and Inner Mongolia Key Laboratory of Sugarbeet Genetics and Germplasm Enhancement, Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot, Inner Mongolia, 010031, People's Republic of China., Wang YL; Zhejiang Key Laboratory of Multiomics and Molecular Enzymology, Yangtze Delta Region Institute of Tsinghua University, Jiaxing, Zhejiang, 314006, People's Republic of China., Xu SM; Key Laboratory of Laparoscopic Technology of Zhejiang Province, Department of General Surgery, Sir Run-Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310016, People's Republic of China. shimingxu@zju.edu.cn.; Institute of Translational Medicine, Zhejiang University School of Medicine, and Zhejiang University Cancer Center, Hangzhou, Zhejiang, 310029, People's Republic of China. shimingxu@zju.edu.cn., Xie AY; Key Laboratory of Laparoscopic Technology of Zhejiang Province, Department of General Surgery, Sir Run-Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310016, People's Republic of China. anyongxie@zju.edu.cn.; Hangzhou Qiantang Hospital, Hangzhou, Zhejiang, 310018, People's Republic of China. anyongxie@zju.edu.cn.; Institute of Translational Medicine, Zhejiang University School of Medicine, and Zhejiang University Cancer Center, Hangzhou, Zhejiang, 310029, People's Republic of China. anyongxie@zju.edu.cn.

Journal Article , 2025

Reprogrammable RNA-targeting CRISPR systems evolved from RNA toxin-antitoxins.

Zilberzwige-Tal S; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; McGovern Institute for Brain Research at MIT, Cambridge, MA 02139, USA; Department of Brain and Cognitive Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA., Altae-Tran H; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; McGovern Institute for Brain Research at MIT, Cambridge, MA 02139, USA; Department of Brain and Cognitive Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA., Kannan S; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; McGovern Institute for Brain Research at MIT, Cambridge, MA 02139, USA; Department of Brain and Cognitive Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA., Wilkinson ME; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; McGovern Institute for Brain Research at MIT, Cambridge, MA 02139, USA; Department of Brain and Cognitive Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA., Vo SC; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; McGovern Institute for Brain Research at MIT, Cambridge, MA 02139, USA; Department of Brain and Cognitive Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA., Strebinger D; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; McGovern Institute for Brain Research at MIT, Cambridge, MA 02139, USA; Department of Brain and Cognitive Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA., Edmonds KK; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; McGovern Institute for Brain Research at MIT, Cambridge, MA 02139, USA; Department of Brain and Cognitive Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA., Yao CJ; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; McGovern Institute for Brain Research at MIT, Cambridge, MA 02139, USA; Department of Brain and Cognitive Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Molecular Cellular Biology, Harvard University, Cambridge, MA, USA., Mears KS; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; McGovern Institute for Brain Research at MIT, Cambridge, MA 02139, USA; Department of Brain and Cognitive Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA., Shmakov SA; National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD, USA., Makarova KS; National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD, USA., Macrae RK; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; McGovern Institute for Brain Research at MIT, Cambridge, MA 02139, USA; Department of Brain and Cognitive Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA., Koonin EV; National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD, USA., Zhang F; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; McGovern Institute for Brain Research at MIT, Cambridge, MA 02139, USA; Department of Brain and Cognitive Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. Electronic address: zhang@broadinstitute.org.

Journal Article , 2025

Kinetic Mechanism for Fidelity of CRISPR-Cas9 Variants.

Hecht AD; Department of Bioengineering, Rice University, Houston, Texas 77005, United States.; Center for Theoretical Biological Physics, Rice University, Houston, Texas 77005, United States.; Rice Synthetic Biology Institute, Rice University, Houston, Texas 77005, United States., Igoshin OA; Department of Bioengineering, Rice University, Houston, Texas 77005, United States.; Center for Theoretical Biological Physics, Rice University, Houston, Texas 77005, United States.; Rice Synthetic Biology Institute, Rice University, Houston, Texas 77005, United States.; Department of BioSciences, Rice University, Houston, Texas 77005, United States.; Department of Chemistry, Rice University, Houston, Texas 77005, United States.

Journal Article , 2025

Cas9-Rep fusion tethers donor DNA in vivo and boosts the efficiency of HDR-mediated genome editing.

Zhou Z; National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China.; Hubei Key Laboratory of Plant Pathology, Huazhong Agricultural University, Wuhan, China., Xiao J; National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China.; Hubei Key Laboratory of Plant Pathology, Huazhong Agricultural University, Wuhan, China., Yin S; National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China.; Hubei Key Laboratory of Plant Pathology, Huazhong Agricultural University, Wuhan, China., Chen Y; National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China.; Hubei Key Laboratory of Plant Pathology, Huazhong Agricultural University, Wuhan, China., Yuan Y; National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China., Zhang J; National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China., Xiong L; National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China., Xie K; National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China.; Hubei Key Laboratory of Plant Pathology, Huazhong Agricultural University, Wuhan, China.

Journal Article , 2025

Circular RNA-mediated inverse prime editing in human cells.

Liang R; New Cornerstone Science Laboratory, Center for Genome Editing, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China., Wang S; New Cornerstone Science Laboratory, Center for Genome Editing, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China., Cai Y; New Cornerstone Science Laboratory, Center for Genome Editing, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, China., Li Z; New Cornerstone Science Laboratory, Center for Genome Editing, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, China., Li KM; College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China., Wei J; New Cornerstone Science Laboratory, Center for Genome Editing, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China., Sun C; New Cornerstone Science Laboratory, Center for Genome Editing, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China., Zhu H; New Cornerstone Science Laboratory, Center for Genome Editing, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China., Chen K; New Cornerstone Science Laboratory, Center for Genome Editing, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China., Gao C; New Cornerstone Science Laboratory, Center for Genome Editing, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China. cxgao@genetics.ac.cn.; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, China. cxgao@genetics.ac.cn.

Journal Article , 2025

Structural and dynamic impacts of single-atom disruptions to guide RNA interactions within the recognition lobe of Geobacillus stearothermophilus Cas9.

Belato HB; Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, United States., Knight AL; Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, United States., D'Ordine AM; Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, United States., Pindi C; Departments of Bioengineering and Chemistry, University of California, Riverside, Riverside, United States., Fan Z; Brown University Transgenic Mouse and Gene Targeting Facility, Providence, United States., Luo J; Brown University Transgenic Mouse and Gene Targeting Facility, Providence, United States., Palermo G; Departments of Bioengineering and Chemistry, University of California, Riverside, Riverside, United States., Jogl G; Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, United States.; Brown University RNA Center, Providence, United States., Lisi GP; Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, United States.; Brown University RNA Center, Providence, United States.

Journal Article , 2025

Efficient and Precise Integration of Large DNA Sequences Using Precise Interstrand Cross-Linking of Long ssDNA and sgRNA.

Li Z; Department of Polymer Science and Engineering, Hefei National Research Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei 230026, China., Li C; Department of Polymer Science and Engineering, Hefei National Research Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei 230026, China., Xiao S; Department of Polymer Science and Engineering, Hefei National Research Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei 230026, China.; State Key Laboratory of Precision and Intelligent Chemistry, University of Science and Technology of China, Hefei, Anhui 230026, China., Liang H; Department of Polymer Science and Engineering, Hefei National Research Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei 230026, China.

Journal Article; Research Support, Non-U.S. Gov't , 2025

In vitro activation of anti-cancer gene expression by delivery of CRISPR/dCas9 ribonucleoproteins to suppress glioblastoma.

Lee MH; Department of Materials Science and Engineering, I-Shou University, Kaohsiung 84001, Taiwan., Thomas JL; Department of Physics and Astronomy, University of New Mexico, Albuquerque, NM 87131, USA., Lin YL; Department of Chemical and Materials Engineering, National University of Kaohsiung, Kaohsiung 81148, Taiwan., Lin HY; Department of Chemical and Materials Engineering, National University of Kaohsiung, Kaohsiung 81148, Taiwan. Electronic address: linhy@ntu.edu.tw.

Journal Article , 2025

Make-or-break prime editing for genome engineering in Streptococcus pneumoniae.

Rengifo-Gonzalez M; Department of Fundamental Microbiology, Faculty of Biology and Medicine, University of Lausanne, Biophore Building, CH-, Lausanne, Switzerland., Mazzuoli MV; Department of Fundamental Microbiology, Faculty of Biology and Medicine, University of Lausanne, Biophore Building, CH-, Lausanne, Switzerland., Janssen AB; Department of Fundamental Microbiology, Faculty of Biology and Medicine, University of Lausanne, Biophore Building, CH-, Lausanne, Switzerland., Rueff AS; Department of Fundamental Microbiology, Faculty of Biology and Medicine, University of Lausanne, Biophore Building, CH-, Lausanne, Switzerland., Burnier J; Department of Fundamental Microbiology, Faculty of Biology and Medicine, University of Lausanne, Biophore Building, CH-, Lausanne, Switzerland., Liu X; Department of Pathogen Biology, Base for International Science and Technology Cooperation: Carson Cancer Stem Cell Vaccines R&D Center, International Cancer Center, Shenzhen University Medical School, Shenzhen, Guangdong, China. xueliu@szu.edu.cn., Veening JW; Department of Fundamental Microbiology, Faculty of Biology and Medicine, University of Lausanne, Biophore Building, CH-, Lausanne, Switzerland. Jan-Willem.Veening@unil.ch.

Journal Article , 2025

CRISPR-CISH: an in situ chromogenic DNA repeat detection system for research and life science education.

Potlapalli BP; Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Corrensstraße 3, 06466, Seeland, Germany. potlapalli@ipk-gatersleben.de., Dassau F; Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Corrensstraße 3, 06466, Seeland, Germany.; Hochschule Mittweida, Department of Biotechnology, 09648, Mittweida, Germany., Fuchs J; Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Corrensstraße 3, 06466, Seeland, Germany., Sushmoy DR; Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Corrensstraße 3, 06466, Seeland, Germany.; Julius Kühn-Institute (JKI), 06484, Quedlinburg, Germany., Houben A; Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Corrensstraße 3, 06466, Seeland, Germany. houben@ipk-gatersleben.de.

Journal Article; Research Support, Non-U.S. Gov't , 2025

An innovative approach using CRISPR-ribonucleoprotein packaged in virus-like particles to generate genetically engineered mouse models.

Jeong TY; Department of Physiology, Korea University College of Medicine, Seoul, Republic of Korea.; Department of Biomedical Sciences, Korea University College of Medicine, Seoul, Republic of Korea.; Laboratory for Genomic and Epigenomic Medicine, Research Institute for Veterinary Science, and BK21 PLUS Program for Creative Veterinary Science Research, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea., Yoon DE; Department of Physiology, Korea University College of Medicine, Seoul, Republic of Korea.; Department of Biomedical Sciences, Korea University College of Medicine, Seoul, Republic of Korea.; Laboratory for Genomic and Epigenomic Medicine, Research Institute for Veterinary Science, and BK21 PLUS Program for Creative Veterinary Science Research, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea.; Transgenic core facility, Max-Planck Institute of Biochemistry, Martinsried, Germany., Kim SP; Korea Model animal Priority Center, Seoul National University, Seoul, Republic of Korea.; Laboratory of Developmental Biology and Genomics, Research Institute for Veterinary Science, and BK21 PLUS Program for Creative Veterinary Science Research, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea., Yang J; Department of Physiology, Korea University College of Medicine, Seoul, Republic of Korea.; Department of Biomedical Sciences, Korea University College of Medicine, Seoul, Republic of Korea.; Laboratory for Genomic and Epigenomic Medicine, Research Institute for Veterinary Science, and BK21 PLUS Program for Creative Veterinary Science Research, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea., Lim SY; Korea Model animal Priority Center, Seoul National University, Seoul, Republic of Korea., Ok S; Department of Physiology, Korea University College of Medicine, Seoul, Republic of Korea., Ju S; Department of Physiology, Korea University College of Medicine, Seoul, Republic of Korea.; Department of Biomedical Sciences, Korea University College of Medicine, Seoul, Republic of Korea.; Laboratory for Genomic and Epigenomic Medicine, Research Institute for Veterinary Science, and BK21 PLUS Program for Creative Veterinary Science Research, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea., Park J; Department of Physiology, Korea University College of Medicine, Seoul, Republic of Korea.; Laboratory for Genomic and Epigenomic Medicine, Research Institute for Veterinary Science, and BK21 PLUS Program for Creative Veterinary Science Research, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea.; Korea Model animal Priority Center, Seoul National University, Seoul, Republic of Korea., Lee SB; Korea Model animal Priority Center, Seoul National University, Seoul, Republic of Korea., Park SJ; Department of Physiology, Korea University College of Medicine, Seoul, Republic of Korea.; Department of Biomedical Sciences, Korea University College of Medicine, Seoul, Republic of Korea.; Laboratory for Genomic and Epigenomic Medicine, Research Institute for Veterinary Science, and BK21 PLUS Program for Creative Veterinary Science Research, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea., Kim S; Department of Biomedical Sciences, Korea University College of Medicine, Seoul, Republic of Korea.; Department of Convergence Medicine, Korea University College of Medicine, Seoul, Republic of Korea., Lee H; Department of Biomedical Sciences, Korea University College of Medicine, Seoul, Republic of Korea.; Department of Convergence Medicine, Korea University College of Medicine, Seoul, Republic of Korea., Lee D; Department of Life Science, Ewha Womans University, Seoul, Republic of Korea., Kang SK; Korea Model animal Priority Center, Seoul National University, Seoul, Republic of Korea.; Laboratory of Developmental Biology and Genomics, Research Institute for Veterinary Science, and BK21 PLUS Program for Creative Veterinary Science Research, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea., Lee SE; Research Animal Resource Center, Korea Institute of Science and Technology (KIST), Seoul, Republic of Korea., Kim HS; Department of Anatomy, Korea University College of Medicine, Seoul, Republic of Korea., Seong JK; Korea Model animal Priority Center, Seoul National University, Seoul, Republic of Korea. snumouse@snu.ac.kr.; Laboratory of Developmental Biology and Genomics, Research Institute for Veterinary Science, and BK21 PLUS Program for Creative Veterinary Science Research, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea. snumouse@snu.ac.kr.; Interdisciplinary Program in Bioinformatics and BIO MAX/N-Bio Institute, Seoul National University, Seoul, Republic of Korea. snumouse@snu.ac.kr.; Interdisciplinary Program of Cancer Biology, Seoul National University Cancer Research Institute, Seoul, Republic of Korea. snumouse@snu.ac.kr., Kim K; Department of Physiology, Korea University College of Medicine, Seoul, Republic of Korea. kyoungmi_kim@snu.ac.kr.; Department of Biomedical Sciences, Korea University College of Medicine, Seoul, Republic of Korea. kyoungmi_kim@snu.ac.kr.; Laboratory for Genomic and Epigenomic Medicine, Research Institute for Veterinary Science, and BK21 PLUS Program for Creative Veterinary Science Research, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea. kyoungmi_kim@snu.ac.kr.; Korea Model animal Priority Center, Seoul National University, Seoul, Republic of Korea. kyoungmi_kim@snu.ac.kr.

Journal Article , 2025

Nonequilibrium hybridization-driven CRISPR/Cas adapter with extended energetic penalty for discrimination of single-nucleotide variants.

Liu Q; Department of Key Laboratory of Chemical Biology & Traditional Chinese Medicine, Research, Ministry of Education, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, P. R. China., Jiang Z; Department of Thoracic Medicine, Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Hunan Cancer Hospital, Changsha 410006, P. R. China., Li S; Department of Key Laboratory of Chemical Biology & Traditional Chinese Medicine, Research, Ministry of Education, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, P. R. China., Li Y; Department of Key Laboratory of Chemical Biology & Traditional Chinese Medicine, Research, Ministry of Education, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, P. R. China., Wan Y; Department of Key Laboratory of Chemical Biology & Traditional Chinese Medicine, Research, Ministry of Education, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, P. R. China., Hu Z; Department of Key Laboratory of Chemical Biology & Traditional Chinese Medicine, Research, Ministry of Education, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, P. R. China., Ma S; Department of Key Laboratory of Chemical Biology & Traditional Chinese Medicine, Research, Ministry of Education, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, P. R. China., Zou Z; Department of Key Laboratory of Chemical Biology & Traditional Chinese Medicine, Research, Ministry of Education, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, P. R. China., Yang R; Department of Key Laboratory of Chemical Biology & Traditional Chinese Medicine, Research, Ministry of Education, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, P. R. China.

Journal Article , 2025

Unveiling Cas8 dynamics and regulation within a transposon-encoded Cascade-TniQ complex.

Patel AC; Department of Bioengineering, University of California, Riverside, CA 52512., Sinha S; Department of Bioengineering, University of California, Riverside, CA 52512., Arantes PR; Department of Bioengineering, University of California, Riverside, CA 52512., Palermo G; Department of Bioengineering, University of California, Riverside, CA 52512.; Department of Chemistry, University of California, Riverside, CA 52512.

Journal Article , 2025

Linking CRISPR-Cas9 double-strand break profiles to gene editing precision with BreakTag.

Longo GMC; Institute of Molecular Biology (IMB), Mainz, Germany., Sayols S; Institute of Molecular Biology (IMB), Mainz, Germany., Kotini AG; Department of Biology, Medical School, University of Patras, Patras, Greece., Heinen S; Institute of Molecular Biology (IMB), Mainz, Germany., Möckel MM; Institute of Molecular Biology (IMB), Mainz, Germany., Beli P; Institute of Molecular Biology (IMB), Mainz, Germany.; Johannes Gutenberg University (JGU), Mainz, Germany., Roukos V; Institute of Molecular Biology (IMB), Mainz, Germany. v.roukos@imb-mainz.de.; Department of Biology, Medical School, University of Patras, Patras, Greece. v.roukos@imb-mainz.de.

Journal Article , 2025

The dCas9/crRNA linked immunological assay (dCLISA) for sensitive, accurate, and facile drug resistance gene analysis.

Fan X; Jinling Clinical Medical College, Nanjing University of Chinese Medicine, 210002, Nanjing, Jiangsu Province, China., Gao Z; Jinling Clinical Medical College, Nanjing University of Chinese Medicine, 210002, Nanjing, Jiangsu Province, China., Ling D; Department of Orthopedics, Jinling Hospital, Affiliated Hospital of Medical School, Nanjing University, 210002, Nanjing, Jiangsu Province, China., Wang D; Department of Orthopedics, Jinling Hospital, Affiliated Hospital of Medical School, Nanjing University, 210002, Nanjing, Jiangsu Province, China., Cui Y; Department of Orthopedics, Jinling Hospital, Affiliated Hospital of Medical School, Nanjing University, 210002, Nanjing, Jiangsu Province, China., Du H; Department of Orthopedics, Jinling Hospital, Affiliated Hospital of Medical School, Nanjing University, 210002, Nanjing, Jiangsu Province, China., Zhou X; Jinling Clinical Medical College, Nanjing University of Chinese Medicine, 210002, Nanjing, Jiangsu Province, China; Department of Orthopedics, Jinling Hospital, Affiliated Hospital of Medical School, Nanjing University, 210002, Nanjing, Jiangsu Province, China; Division of Trauma and Acute Care Surgery, Department of Surgery, Jinling Hospital, Jinling Clinical Medical College, Nanjing University of Chinese Medicine, 210002, Nanjing, Jiangsu Province, China. Electronic address: dr_zhouxing@126.com.

Journal Article , 2025

Recent advances in spatiotemporal control of the CRISPR/Cas9 system.

Zhang J; School of Life Science and Technology, Wuhan Polytechnic University, Wuhan, Hubei 430023, China; School of Life Sciences, State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei University, Wuhan, Hubei 430042, China., Zhou Y; School of Life Science and Technology, Wuhan Polytechnic University, Wuhan, Hubei 430023, China., Qiao J; School of Life Science and Technology, Wuhan Polytechnic University, Wuhan, Hubei 430023, China. Electronic address: jieqiao@whpu.edu.cn., Liu Y; School of Life Sciences, State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei University, Wuhan, Hubei 430042, China. Electronic address: yiliu0825@hubu.edu.cn.

Journal Article; Review , 2025