Sökresultat - "CRISPR/Cas"

Precision multiplexed base editing in human cells using Cas12a-derived base editors.

Schweitzer AY; Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT, USA.; Systems Biology Institute, Yale University, West Haven, CT, USA., Adams EW; Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT, USA.; Systems Biology Institute, Yale University, West Haven, CT, USA., Nguyen MTA; Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT, USA.; Systems Biology Institute, Yale University, West Haven, CT, USA., Lek M; Department of Genetics, Yale School of Medicine, New Haven, CT, USA., Isaacs FJ; Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT, USA. farren.isaacs@yale.edu.; Systems Biology Institute, Yale University, West Haven, CT, USA. farren.isaacs@yale.edu.; Department of Biomedical Engineering, Yale University, New Haven, CT, USA. farren.isaacs@yale.edu.

Journal Article , 2025

Structural and dynamic impacts of single-atom disruptions to guide RNA interactions within the recognition lobe of Geobacillus stearothermophilus Cas9.

Belato HB; Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, United States., Knight AL; Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, United States., D'Ordine AM; Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, United States., Pindi C; Departments of Bioengineering and Chemistry, University of California, Riverside, Riverside, United States., Fan Z; Brown University Transgenic Mouse and Gene Targeting Facility, Providence, United States., Luo J; Brown University Transgenic Mouse and Gene Targeting Facility, Providence, United States., Palermo G; Departments of Bioengineering and Chemistry, University of California, Riverside, Riverside, United States., Jogl G; Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, United States.; Brown University RNA Center, Providence, United States., Lisi GP; Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, United States.; Brown University RNA Center, Providence, United States.

Journal Article , 2025

Efficient and Precise Integration of Large DNA Sequences Using Precise Interstrand Cross-Linking of Long ssDNA and sgRNA.

Li Z; Department of Polymer Science and Engineering, Hefei National Research Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei 230026, China., Li C; Department of Polymer Science and Engineering, Hefei National Research Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei 230026, China., Xiao S; Department of Polymer Science and Engineering, Hefei National Research Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei 230026, China.; State Key Laboratory of Precision and Intelligent Chemistry, University of Science and Technology of China, Hefei, Anhui 230026, China., Liang H; Department of Polymer Science and Engineering, Hefei National Research Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei 230026, China.

Journal Article; Research Support, Non-U.S. Gov't , 2025

Characterization and Full Sequencing of 100 Nt sgRNA and Large RNA Using Site-Directed Cleavage and Liquid Chromatography Tandem Mass Spectrometry.

Yan H; Nucleic Acid R&D, RSBU LSSII, GenScript Biotech Corporation, 28 Yongxi Street, Nanjing 211100, Jiangsu, China., Qi M; Nucleic Acid R&D, RSBU LSSII, GenScript Biotech Corporation, 28 Yongxi Street, Nanjing 211100, Jiangsu, China., Li H; Nucleic Acid R&D, RSBU LSSII, GenScript Biotech Corporation, 28 Yongxi Street, Nanjing 211100, Jiangsu, China.

Journal Article , 2025

Reprogrammable RNA-targeting CRISPR systems evolved from RNA toxin-antitoxins.

Zilberzwige-Tal S; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; McGovern Institute for Brain Research at MIT, Cambridge, MA 02139, USA; Department of Brain and Cognitive Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA., Altae-Tran H; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; McGovern Institute for Brain Research at MIT, Cambridge, MA 02139, USA; Department of Brain and Cognitive Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA., Kannan S; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; McGovern Institute for Brain Research at MIT, Cambridge, MA 02139, USA; Department of Brain and Cognitive Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA., Wilkinson ME; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; McGovern Institute for Brain Research at MIT, Cambridge, MA 02139, USA; Department of Brain and Cognitive Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA., Vo SC; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; McGovern Institute for Brain Research at MIT, Cambridge, MA 02139, USA; Department of Brain and Cognitive Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA., Strebinger D; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; McGovern Institute for Brain Research at MIT, Cambridge, MA 02139, USA; Department of Brain and Cognitive Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA., Edmonds KK; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; McGovern Institute for Brain Research at MIT, Cambridge, MA 02139, USA; Department of Brain and Cognitive Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA., Yao CJ; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; McGovern Institute for Brain Research at MIT, Cambridge, MA 02139, USA; Department of Brain and Cognitive Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Molecular Cellular Biology, Harvard University, Cambridge, MA, USA., Mears KS; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; McGovern Institute for Brain Research at MIT, Cambridge, MA 02139, USA; Department of Brain and Cognitive Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA., Shmakov SA; National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD, USA., Makarova KS; National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD, USA., Macrae RK; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; McGovern Institute for Brain Research at MIT, Cambridge, MA 02139, USA; Department of Brain and Cognitive Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA., Koonin EV; National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD, USA., Zhang F; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; McGovern Institute for Brain Research at MIT, Cambridge, MA 02139, USA; Department of Brain and Cognitive Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. Electronic address: zhang@broadinstitute.org.

Journal Article , 2025

Engineering stimuli-responsive CRISPR-Cas systems for versatile biosensing.

Cao L; State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan Provincial Key Laboratory of Biomacromolecular Chemical Biology, Hunan University, Changsha, 410082, Hunan, China., Chen W; State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan Provincial Key Laboratory of Biomacromolecular Chemical Biology, Hunan University, Changsha, 410082, Hunan, China., Kang W; Key Laboratory of Tropical Medicinal Resource Chemistry of Ministry of Education & Laboratory of Tropical Medicinal Plant Chemistry of Hainan Province, College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou, 571158, Hainan, China., Lei C; State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan Provincial Key Laboratory of Biomacromolecular Chemical Biology, Hunan University, Changsha, 410082, Hunan, China. cylei@hnu.edu.cn., Nie Z; State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan Provincial Key Laboratory of Biomacromolecular Chemical Biology, Hunan University, Changsha, 410082, Hunan, China. niezhou.hnu@gmail.com.

Journal Article; Review , 2025

Recent advances in spatiotemporal control of the CRISPR/Cas9 system.

Zhang J; School of Life Science and Technology, Wuhan Polytechnic University, Wuhan, Hubei 430023, China; School of Life Sciences, State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei University, Wuhan, Hubei 430042, China., Zhou Y; School of Life Science and Technology, Wuhan Polytechnic University, Wuhan, Hubei 430023, China., Qiao J; School of Life Science and Technology, Wuhan Polytechnic University, Wuhan, Hubei 430023, China. Electronic address: jieqiao@whpu.edu.cn., Liu Y; School of Life Sciences, State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei University, Wuhan, Hubei 430042, China. Electronic address: yiliu0825@hubu.edu.cn.

Journal Article; Review , 2025

Trans-nuclease activity of Cas9 activated by DNA or RNA target binding.

Chen J; State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Faculty of Medicine and Life Sciences, Xiamen University, Xiamen, China., Chen Y; State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Faculty of Medicine and Life Sciences, Xiamen University, Xiamen, China., Huang L; State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Faculty of Medicine and Life Sciences, Xiamen University, Xiamen, China., Lin X; State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Faculty of Medicine and Life Sciences, Xiamen University, Xiamen, China., Chen H; State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Faculty of Medicine and Life Sciences, Xiamen University, Xiamen, China., Xiang W; State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Faculty of Medicine and Life Sciences, Xiamen University, Xiamen, China., Liu L; State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Faculty of Medicine and Life Sciences, Xiamen University, Xiamen, China. liangliu2019@xmu.edu.cn.

Journal Article , 2025

Optimized CRISPR-based knockout in BeWo cells.

Yin E; University of California, Berkeley, United States., Esbin MN; University of California, Berkeley, United States. Electronic address: meagan_esbin@berkeley.edu.

Journal Article , 2025

A versatile CRISPR/Cas9 system off-target prediction tool using language model.

Du W; Gene Editing Technology Center of Guangdong Province, School of Medicine, Foshan University, Foshan, Guangdong, China., Zhao L; Shenzhen Health Development Research and Data Management Center, Shenzhen, Guangdong, China. zhaoj93@mail2.sysu.edu.cn., Diao K; Shenzhen Center for Chronic Disease Control, Shenzhen, Guangdong, China., Zheng Y; Guangdong Homy Genetics Ltd, Foshan, Guangdong, China., Yang Q; Jiujiang Key Laboratory of Rare Disease Research, Jiujiang University, Jiujiang, Jiangxi, China., Zhu Z; Shenzhen Health Development Research and Data Management Center, Shenzhen, Guangdong, China., Zhu X; Gene Editing Technology Center of Guangdong Province, School of Medicine, Foshan University, Foshan, Guangdong, China. zhu_xiangxing@126.com.; Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, Foshan University, Foshan, Guangdong, China. zhu_xiangxing@126.com., Tang D; Gene Editing Technology Center of Guangdong Province, School of Medicine, Foshan University, Foshan, Guangdong, China. tangdsh@163.com.; Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, Foshan University, Foshan, Guangdong, China. tangdsh@163.com.

Journal Article , 2025

Kinetic Mechanism for Fidelity of CRISPR-Cas9 Variants.

Hecht AD; Department of Bioengineering, Rice University, Houston, Texas 77005, United States.; Center for Theoretical Biological Physics, Rice University, Houston, Texas 77005, United States.; Rice Synthetic Biology Institute, Rice University, Houston, Texas 77005, United States., Igoshin OA; Department of Bioengineering, Rice University, Houston, Texas 77005, United States.; Center for Theoretical Biological Physics, Rice University, Houston, Texas 77005, United States.; Rice Synthetic Biology Institute, Rice University, Houston, Texas 77005, United States.; Department of BioSciences, Rice University, Houston, Texas 77005, United States.; Department of Chemistry, Rice University, Houston, Texas 77005, United States.

Journal Article , 2025

Assessment of Miniature AsCas12f1 Variants for Gene Editing and Activation.

Ren C; Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, Zhejiang, China.; ZJU-Hangzhou Global Scientific and Technological Innovation Center, Zhejiang University, Hangzhou, Zhejiang, China.; Institute of Bioengineering, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, Zhejiang, China., Bao Z; Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, Zhejiang, China.; ZJU-Hangzhou Global Scientific and Technological Innovation Center, Zhejiang University, Hangzhou, Zhejiang, China.; Institute of Bioengineering, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, Zhejiang, China.; Zhejiang Key Laboratory of Smart Biomaterials, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, Zhejiang, China.

Journal Article , 2025

Highly efficient Agrobacterium rhizogenes-mediated gene editing system in Salvia miltiorrhiza inbred line bh2-7.

Tian M; State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijng, China., Luo L; State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijng, China., Jin B; State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijng, China., Liu J; State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijng, China., Chen T; State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijng, China., Tang J; State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijng, China., Shen Y; State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijng, China., Zhang H; State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijng, China., Guo J; State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijng, China., Zhang H; National Key Laboratory of Wheat Improvement, Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agriculture Sciences in Weifang, Weifang, Shandong, China., Cui G; State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijng, China., Huang L; State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijng, China.

Journal Article , 2025

Circular RNA-mediated inverse prime editing in human cells.

Liang R; New Cornerstone Science Laboratory, Center for Genome Editing, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China., Wang S; New Cornerstone Science Laboratory, Center for Genome Editing, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China., Cai Y; New Cornerstone Science Laboratory, Center for Genome Editing, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, China., Li Z; New Cornerstone Science Laboratory, Center for Genome Editing, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, China., Li KM; College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China., Wei J; New Cornerstone Science Laboratory, Center for Genome Editing, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China., Sun C; New Cornerstone Science Laboratory, Center for Genome Editing, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China., Zhu H; New Cornerstone Science Laboratory, Center for Genome Editing, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China., Chen K; New Cornerstone Science Laboratory, Center for Genome Editing, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China., Gao C; New Cornerstone Science Laboratory, Center for Genome Editing, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China. cxgao@genetics.ac.cn.; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, China. cxgao@genetics.ac.cn.

Journal Article , 2025

Generation and validation of a versatile inducible multiplex CRISPRi system to examine bacterial regulation in the Euprymna-Vibrio fischeri symbiosis.

Pipes BL; Department of Molecular and Cell Biology, University of California, Merced, CA, 95343, USA., Nishiguchi MK; Department of Molecular and Cell Biology, University of California, Merced, CA, 95343, USA. nish@ucmerced.edu.

Journal Article , 2025

Array Assembler Provides Greatly Simplified crRNA Array Design for CRISPR Cas12 and Cas13 Variants.

Bryson JW; Biotech Research and Innovation Centre (BRIC), University of Copenhagen, Copenhagen 2200, Denmark.

Journal Article; Research Support, Non-U.S. Gov't , 2025

Programmable gene insertion in human cells with a laboratory-evolved CRISPR-associated transposase.

Witte IP; Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of Harvard and MIT, Cambridge, MA, USA.; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA.; Howard Hughes Medical Institute, Harvard University, Cambridge, MA, USA., Lampe GD; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA.; Howard Hughes Medical Institute, Columbia University, New York, NY, USA., Eitzinger S; Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of Harvard and MIT, Cambridge, MA, USA.; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA.; Howard Hughes Medical Institute, Harvard University, Cambridge, MA, USA., Miller SM; Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of Harvard and MIT, Cambridge, MA, USA.; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA.; Howard Hughes Medical Institute, Harvard University, Cambridge, MA, USA., Berríos KN; Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of Harvard and MIT, Cambridge, MA, USA.; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA.; Howard Hughes Medical Institute, Harvard University, Cambridge, MA, USA., McElroy AN; Department of Pediatrics, University of Minnesota Medical School, Minneapolis, MN, USA., King RT; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA., Stringham OG; Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of Harvard and MIT, Cambridge, MA, USA.; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA.; Howard Hughes Medical Institute, Harvard University, Cambridge, MA, USA., Gelsinger DR; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA., Vo PLH; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA., Chen AT; Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of Harvard and MIT, Cambridge, MA, USA.; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA.; Howard Hughes Medical Institute, Harvard University, Cambridge, MA, USA., Tolar J; Department of Pediatrics, University of Minnesota Medical School, Minneapolis, MN, USA., Osborn MJ; Department of Pediatrics, University of Minnesota Medical School, Minneapolis, MN, USA., Sternberg SH; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA.; Howard Hughes Medical Institute, Columbia University, New York, NY, USA., Liu DR; Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of Harvard and MIT, Cambridge, MA, USA.; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA.; Howard Hughes Medical Institute, Harvard University, Cambridge, MA, USA.

Journal Article , 2025

Construction of multi-targeted CRISPR libraries in tomato to overcome functional redundancy at genome-scale level.

Berman A; School of Plant Sciences and Food Security, Tel Aviv University, Tel Aviv, Israel., Su N; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, China., Li Z; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, China., Landau U; School of Plant Sciences and Food Security, Tel Aviv University, Tel Aviv, Israel., Chakraborty J; School of Plant Sciences and Food Security, Tel Aviv University, Tel Aviv, Israel., Gerbi N; School of Plant Sciences and Food Security, Tel Aviv University, Tel Aviv, Israel., Liu J; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, China., Qin Y; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, China., Yuan B; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, China., Wei W; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, China.; Key Lab of Seed Innovation, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China., Yanai O; NetaGenomiX, Netter Center, Mikveh Israel, Israel., Mayrose I; School of Plant Sciences and Food Security, Tel Aviv University, Tel Aviv, Israel., Zhang Y; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, China. zhangyuqin@ucas.ac.cn., Shani E; School of Plant Sciences and Food Security, Tel Aviv University, Tel Aviv, Israel. eilonsh@tauex.tau.ac.il.

Journal Article , 2025

Tracking-seq reveals the heterogeneity of off-target effects in CRISPR-Cas9-mediated genome editing.

Zhu M; Tsinghua-Peking Joint Center for Life Sciences, Tsinghua University, Beijing, China. zhu-m16@tsinghua.org.cn.; Department of Basic Medical Sciences, School of Medicine, Tsinghua University, Beijing, China. zhu-m16@tsinghua.org.cn.; MOE Key Laboratory of Bioinformatics, State Key Laboratory of Molecular Oncology, Tsinghua University, Beijing, China. zhu-m16@tsinghua.org.cn., Xu R; Tsinghua-Peking Joint Center for Life Sciences, Tsinghua University, Beijing, China.; Department of Basic Medical Sciences, School of Medicine, Tsinghua University, Beijing, China.; MOE Key Laboratory of Bioinformatics, State Key Laboratory of Molecular Oncology, Tsinghua University, Beijing, China., Yuan J; Tsinghua-Peking Joint Center for Life Sciences, Tsinghua University, Beijing, China.; IDG-McGovern Institute for Brain Research, Center for Synthetic and Systems Biology, School of Pharmaceutical Sciences, Tsinghua University, Beijing, China., Wang J; Tsinghua-Peking Joint Center for Life Sciences, Tsinghua University, Beijing, China.; MOE Key Laboratory of Bioinformatics, State Key Laboratory of Molecular Oncology, Tsinghua University, Beijing, China.; School of Life Sciences, Tsinghua University, Beijing, China., Ren X; Tsinghua-Peking Joint Center for Life Sciences, Tsinghua University, Beijing, China.; IDG-McGovern Institute for Brain Research, Center for Synthetic and Systems Biology, School of Pharmaceutical Sciences, Tsinghua University, Beijing, China., Cong T; Tsinghua-Peking Joint Center for Life Sciences, Tsinghua University, Beijing, China.; IDG-McGovern Institute for Brain Research, Center for Synthetic and Systems Biology, School of Pharmaceutical Sciences, Tsinghua University, Beijing, China., You Y; Tsinghua-Peking Joint Center for Life Sciences, Tsinghua University, Beijing, China.; Department of Basic Medical Sciences, School of Medicine, Tsinghua University, Beijing, China.; MOE Key Laboratory of Bioinformatics, State Key Laboratory of Molecular Oncology, Tsinghua University, Beijing, China., Ju A; Tsinghua-Peking Joint Center for Life Sciences, Tsinghua University, Beijing, China.; Department of Basic Medical Sciences, School of Medicine, Tsinghua University, Beijing, China.; MOE Key Laboratory of Bioinformatics, State Key Laboratory of Molecular Oncology, Tsinghua University, Beijing, China., Xu L; Tsinghua-Peking Joint Center for Life Sciences, Tsinghua University, Beijing, China.; School of Life Sciences, Tsinghua University, Beijing, China., Wang H; Tsinghua-Peking Joint Center for Life Sciences, Tsinghua University, Beijing, China.; Department of Basic Medical Sciences, School of Medicine, Tsinghua University, Beijing, China., Zheng P; Tsinghua-Peking Joint Center for Life Sciences, Tsinghua University, Beijing, China.; IDG-McGovern Institute for Brain Research, Center for Synthetic and Systems Biology, School of Pharmaceutical Sciences, Tsinghua University, Beijing, China., Tao H; Department of Basic Medical Sciences, School of Medicine, Tsinghua University, Beijing, China.; Department of Urology, Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, China., Lin C; Department of Urology, Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, China., Yu H; Department of Basic Medical Sciences, School of Medicine, Tsinghua University, Beijing, China.; Key Laboratory of Medical Biotechnology and Translational Medicine, Guilin Medical University, Guilin, China., Du J; Tsinghua-Peking Joint Center for Life Sciences, Tsinghua University, Beijing, China.; IDG-McGovern Institute for Brain Research, Center for Synthetic and Systems Biology, School of Pharmaceutical Sciences, Tsinghua University, Beijing, China., Lin X; Tsinghua-Peking Joint Center for Life Sciences, Tsinghua University, Beijing, China.; Department of Basic Medical Sciences, School of Medicine, Tsinghua University, Beijing, China., Xie W; Tsinghua-Peking Joint Center for Life Sciences, Tsinghua University, Beijing, China.; School of Life Sciences, Tsinghua University, Beijing, China., Li Y; MOE Key Laboratory of Bioinformatics, State Key Laboratory of Molecular Oncology, Tsinghua University, Beijing, China. yinqingl@tsinghua.edu.cn.; IDG-McGovern Institute for Brain Research, Center for Synthetic and Systems Biology, School of Pharmaceutical Sciences, Tsinghua University, Beijing, China. yinqingl@tsinghua.edu.cn., Lan X; Tsinghua-Peking Joint Center for Life Sciences, Tsinghua University, Beijing, China. xlan@mail.tsinghua.edu.cn.; Department of Basic Medical Sciences, School of Medicine, Tsinghua University, Beijing, China. xlan@mail.tsinghua.edu.cn.; MOE Key Laboratory of Bioinformatics, State Key Laboratory of Molecular Oncology, Tsinghua University, Beijing, China. xlan@mail.tsinghua.edu.cn.

Journal Article , 2025

Viral delivery of an RNA-guided genome editor for transgene-free germline editing in Arabidopsis.

Weiss T; Department of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA, USA., Kamalu M; Department of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA, USA., Shi H; Innovative Genomics Institute, University of California, Berkeley, CA, USA.; Howard Hughes Medical Institute, University of California, Berkeley, CA, USA., Li Z; Department of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA, USA., Amerasekera J; Department of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA, USA., Zhong Z; Department of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA, USA.; Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment, College of Life Sciences, State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu, China., Adler BA; Innovative Genomics Institute, University of California, Berkeley, CA, USA.; California Institute for Quantitative Biosciences (QB3), University of California, Berkeley, CA, USA., Song MM; Department of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA, USA., Vohra K; Innovative Genomics Institute, University of California, Berkeley, CA, USA.; California Institute for Quantitative Biosciences (QB3), University of California, Berkeley, CA, USA., Wirnowski G; Department of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA, USA., Chitkara S; Department of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA, USA., Ambrose C; Department of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA, USA., Steinmetz N; Department of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA, USA., Sridharan A; Department of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA, USA., Sahagun D; Department of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA, USA., Banfield JF; Innovative Genomics Institute, University of California, Berkeley, CA, USA.; Department of Earth and Planetary Science, University of California, Berkeley, CA, USA.; Department of Environmental Science, Policy and Management, University of California, Berkeley, CA, USA.; University of Melbourne, Melbourne, Australia., Doudna JA; Innovative Genomics Institute, University of California, Berkeley, CA, USA.; Howard Hughes Medical Institute, University of California, Berkeley, CA, USA.; California Institute for Quantitative Biosciences (QB3), University of California, Berkeley, CA, USA.; Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA.; Department of Chemistry, University of California, Berkeley, CA, USA.; Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.; Li Ka Shing Center for Translational Genomics, University of California, Berkeley, CA, USA.; Gladstone Institute of Data Science and Biotechnology, San Francisco, CA, USA.; Gladstone-UCSF Institute of Genomic Immunology, San Francisco, CA, USA., Jacobsen SE; Department of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA, USA. jacobsen@ucla.edu.; Howard Hughes Medical Institute (HHMI), University of California at Los Angeles, Los Angeles, CA, USA. jacobsen@ucla.edu.

Journal Article , 2025