Sökresultat - "Decalcification"

One protocol to rule them all: a pilot study to identify the best fixation and decalcification approach for bone marrow biopsy immunohistochemistry.

Paudice M; IRCCS San Martino Policlinic Hospital of Genoa, Genoa, Italy.; Pathology Unit, Department of Surgical Sciences and Integrated Diagnostics (DISC), University of Genoa, Genoa, Italy., Gambella A; Pathology Unit, Department of Surgical Sciences and Integrated Diagnostics (DISC), University of Genoa, Genoa, Italy., Pigozzi S; IRCCS San Martino Policlinic Hospital of Genoa, Genoa, Italy.; Pathology Unit, Department of Surgical Sciences and Integrated Diagnostics (DISC), University of Genoa, Genoa, Italy., Campora M; Department of Pathology, Santa Chiara Hospital, APSS Trento, Trento, Italy., Pitto F; IRCCS San Martino Policlinic Hospital of Genoa, Genoa, Italy.; Pathology Unit, Department of Surgical Sciences and Integrated Diagnostics (DISC), University of Genoa, Genoa, Italy., Bozzano S; Pathology Unit, Department of Surgical Sciences and Integrated Diagnostics (DISC), University of Genoa, Genoa, Italy., Mastracci L; IRCCS San Martino Policlinic Hospital of Genoa, Genoa, Italy.; Pathology Unit, Department of Surgical Sciences and Integrated Diagnostics (DISC), University of Genoa, Genoa, Italy., Grillo F; IRCCS San Martino Policlinic Hospital of Genoa, Genoa, Italy.; Pathology Unit, Department of Surgical Sciences and Integrated Diagnostics (DISC), University of Genoa, Genoa, Italy.

Journal Article , 2025

Resolving the bone - optimizing decalcification in spatial transcriptomics and molecular pathology.

Wang S; Department of Pathology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.; Cancer Program, Broad Institute of MIT and Harvard, Cambridge, MA, USA.

Journal Article; Review , 2025

Qualitative comparison of decalcifiers for mouse bone cryosections for subsequent biophotonic analysis.

Mandal S; Leibniz Institute of Photonic Technology (Member of Leibniz Health Technologies, Member of the Leibniz Centre for Photonics in Infection Research, LPI), 07745, Jena, Germany., Motganhalli Ravikumar R; Leibniz Institute of Photonic Technology (Member of Leibniz Health Technologies, Member of the Leibniz Centre for Photonics in Infection Research, LPI), 07745, Jena, Germany., Tannert A; Leibniz Institute of Photonic Technology (Member of Leibniz Health Technologies, Member of the Leibniz Centre for Photonics in Infection Research, LPI), 07745, Jena, Germany.; Center for Sepsis Control and Care, Jena University Hospital, 07747, Jena, Germany., Urbanek A; Leibniz Institute of Photonic Technology (Member of Leibniz Health Technologies, Member of the Leibniz Centre for Photonics in Infection Research, LPI), 07745, Jena, Germany., Guliev RR; Leibniz Institute of Photonic Technology (Member of Leibniz Health Technologies, Member of the Leibniz Centre for Photonics in Infection Research, LPI), 07745, Jena, Germany., Naumann M; Leibniz Institute of Photonic Technology (Member of Leibniz Health Technologies, Member of the Leibniz Centre for Photonics in Infection Research, LPI), 07745, Jena, Germany.; Center for Sepsis Control and Care, Jena University Hospital, 07747, Jena, Germany.; Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University Jena, 07743, Jena, Germany., Coldewey SM; Center for Sepsis Control and Care, Jena University Hospital, 07747, Jena, Germany.; Department of Anesthesiology and Intensive Care Medicine, Jena University Hospital, 07747, Jena, Germany.; Septomics Research Center, Jena University Hospital, 07745, Jena, Germany., Dahmen U; Experimental Surgery, Clinic for General, Visceral and Vascular Surgery, Jena University Hospital, 07747, Jena, Germany., Carvalho L; Institute of Anatomical and Molecular Pathology, Faculty of Medicine, University of Coimbra, Coimbra, 3004-504, Portugal., Bastião Silva L; BMD Software, PCI-Creative Science Park, Ílhavo, 3830-352, Portugal., Neugebauer U; Leibniz Institute of Photonic Technology (Member of Leibniz Health Technologies, Member of the Leibniz Centre for Photonics in Infection Research, LPI), 07745, Jena, Germany. ute.neugebauer@uni-jena.de.; Center for Sepsis Control and Care, Jena University Hospital, 07747, Jena, Germany. ute.neugebauer@uni-jena.de.; Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University Jena, 07743, Jena, Germany. ute.neugebauer@uni-jena.de.

Journal Article; Comparative Study; Research Support, Non-U.S. Gov't , 2025

[Introduction of a decalcification method for bone marrow biopsy tissue].

Peng XW; State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China Tianjin Institutes of Health Science, Tianjin 301600, China., Xian M; State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China Tianjin Institutes of Health Science, Tianjin 301600, China., Ban NP; State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China Tianjin Institutes of Health Science, Tianjin 301600, China., Sun Q; State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China Tianjin Institutes of Health Science, Tianjin 301600, China.

Journal Article , 2024

Contrast-Enhanced Micro-CT Imaging of Murine Mandibles: A Multi-Method Approach for Simultaneous Hard and Soft Tissue Analysis.

Hildebrand T; Department of Biomaterials, Institute of Clinical Dentistry, Faculty of Dentistry, University of Oslo, Oslo, Norway.; Oral Research Laboratory, Institute of Clinical Dentistry, Faculty of Dentistry, University of Oslo, Oslo, Norway., Humphris Y; Department of Biomaterials, Institute of Clinical Dentistry, Faculty of Dentistry, University of Oslo, Oslo, Norway.; Faculty of Mechanical Engineering, Ostbayerische Technische Hochschule Regensburg, Regensburg, Germany., Haugen HJ; Department of Biomaterials, Institute of Clinical Dentistry, Faculty of Dentistry, University of Oslo, Oslo, Norway., Nogueira LP; Oral Research Laboratory, Institute of Clinical Dentistry, Faculty of Dentistry, University of Oslo, Oslo, Norway.

Journal Article; Comparative Study , 2025

Spatial proteomics and transcriptomics characterization of tissue and multiple cancer types including decalcified marrow.

Yeung CC; Translational Science and Therapeutics Division, Fred Hutchinson Cancer Center, Seattle, WA, USA.; Department of Laboratory Medicine and Pathology, University of Washington, Seattle, WA, USA., Jones DC; Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Center, Seattle, WA, USA.; Immunotherapy Integrated Research Center (I-IRC), Fred Hutchinson Cancer Center, Seattle, WA, USA., Woolston DW; Translational Science and Therapeutics Division, Fred Hutchinson Cancer Center, Seattle, WA, USA., Seaton B; Translational Science and Therapeutics Division, Fred Hutchinson Cancer Center, Seattle, WA, USA., Donato EL; Translational Science and Therapeutics Division, Fred Hutchinson Cancer Center, Seattle, WA, USA., Lin M; Translational Science and Therapeutics Division, Fred Hutchinson Cancer Center, Seattle, WA, USA., Backman C; Translational Science and Therapeutics Division, Fred Hutchinson Cancer Center, Seattle, WA, USA., Oehler V; Translational Science and Therapeutics Division, Fred Hutchinson Cancer Center, Seattle, WA, USA., Robinson KL; Translational Science and Therapeutics Division, Fred Hutchinson Cancer Center, Seattle, WA, USA., Shimp K; Translational Science and Therapeutics Division, Fred Hutchinson Cancer Center, Seattle, WA, USA., Kulikauskas R; Department of Dermatology, University of Washington, Seattle, WA, USA., Long AN; Immunotherapy Integrated Research Center (I-IRC), Fred Hutchinson Cancer Center, Seattle, WA, USA., Sowerby D; Immunotherapy Integrated Research Center (I-IRC), Fred Hutchinson Cancer Center, Seattle, WA, USA., Elz AE; Immunotherapy Integrated Research Center (I-IRC), Fred Hutchinson Cancer Center, Seattle, WA, USA., Smythe KS; Translational Science and Therapeutics Division, Fred Hutchinson Cancer Center, Seattle, WA, USA., Newell EW; Department of Laboratory Medicine and Pathology, University of Washington, Seattle, WA, USA.; Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Center, Seattle, WA, USA.; Immunotherapy Integrated Research Center (I-IRC), Fred Hutchinson Cancer Center, Seattle, WA, USA.

Journal Article , 2025

A set of pretreatment reagents including improved formula fixation and decalcification facilitating immunohistochemistry and DNA analyses of formalin-fixed paraffin-embedded bone marrow trephine biopsy.

Sun T; Department of Pathology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China., Xu L; Department of Pathology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China., Yao H; Department of Pathology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China., Zhao J; Department of Pathology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China., Chen Z; Department of Pathology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China., Chen Z; Department of Science and Development, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China., Wang B; Department of Pathology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China., Ding W; Department of Pathology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China. Electronic address: 1181010@zju.edu.cn.

Journal Article , 2024

Novel processing and staining methodology of bottlenose dolphin ( Tursiops truncatus) teeth for age determination.

Barrett CE; Global Center for Aquatic Health and Food Security, Mississippi State University, Mississippi, MS, USA., Evans I; Global Center for Aquatic Health and Food Security, Mississippi State University, Mississippi, MS, USA., Morgan T; Department of Pathobiology and Population Medicine, Mississippi State University, Mississippi, MS, USA., Olivier AK; Department of Pathobiology and Population Medicine, Mississippi State University, Mississippi, MS, USA.

Journal Article , 2024

Hypertonic saline- and detergent-accelerated EDTA-based decalcification better preserves mRNA of bones.

Li Z; Advancecor GmbH, Lochhamerstr. 29 A, 82152, Martinsried, Germany. li@advancecor.com., Wenhart C; Advancecor GmbH, Lochhamerstr. 29 A, 82152, Martinsried, Germany., Reimann A; Advancecor GmbH, Lochhamerstr. 29 A, 82152, Martinsried, Germany., Cho YL; Advancecor GmbH, Lochhamerstr. 29 A, 82152, Martinsried, Germany., Adler K; Advancecor GmbH, Lochhamerstr. 29 A, 82152, Martinsried, Germany., Muench G; Advancecor GmbH, Lochhamerstr. 29 A, 82152, Martinsried, Germany.

Journal Article , 2024

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(Immuno)histological Analysis of Ewing Sarcoma

Marcilla, David^Aff4, Machado, Isidro^{Aff5, Aff6}, Grünewald, Thomas G. P.^Aff7, Llombart-Bosch, Antonio^Aff8, de Álava, Enrique^{Aff4, Aff9}

Cidre-Aranaz, Florencia^Aff2, G. P. Grünewald, Thomas^Aff3, Walker, John M.^Aff1

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Expediting the sampling, decalcification, and forensic DNA analysis of large elephant ivory seizures to aid investigations and prosecutions.

Ewart KM; TRACE Wildlife Forensics Network, Edinburgh, Scotland, United Kingdom. Electronic address: kyle.ewart@tracenetwork.org., Lightson AL; TRACE Wildlife Forensics Network, Edinburgh, Scotland, United Kingdom., Sitam FT; National Wildlife Forensic Laboratory, Department of Wildlife and National Parks (PERHILITAN), Kuala Lumpur, Malaysia., Rovie-Ryan JJ; National Wildlife Forensic Laboratory, Department of Wildlife and National Parks (PERHILITAN), Kuala Lumpur, Malaysia., Mather N; Chalmers St, Redfern, Sydney, Australia., McEwing R; TRACE Wildlife Forensics Network, Edinburgh, Scotland, United Kingdom.

Journal Article; Research Support, U.S. Gov't, Non-P.H.S. , 2020

Histological, Histomorphometrical, and Biomechanical Studies of Bone-Implanted Medical Devices: Hard Resin Embedding.

Maglio M; IRCCS Istituto Ortopedico Rizzoli, Laboratory of Preclinical and Surgical Studies, Bologna, Italy., Salamanna F; IRCCS Istituto Ortopedico Rizzoli, Laboratory of Preclinical and Surgical Studies, Bologna, Italy., Brogini S; IRCCS Istituto Ortopedico Rizzoli, Laboratory of Preclinical and Surgical Studies, Bologna, Italy., Borsari V; IRCCS Istituto Ortopedico Rizzoli, Laboratory of Preclinical and Surgical Studies, Bologna, Italy., Pagani S; IRCCS Istituto Ortopedico Rizzoli, Laboratory of Preclinical and Surgical Studies, Bologna, Italy., Nicoli Aldini N; IRCCS Istituto Ortopedico Rizzoli, Laboratory of Preclinical and Surgical Studies, Bologna, Italy., Giavaresi G; IRCCS Istituto Ortopedico Rizzoli, Laboratory of Preclinical and Surgical Studies, Bologna, Italy., Fini M; IRCCS Istituto Ortopedico Rizzoli, Laboratory of Biomechanics and Technology Innovation, Bologna, Italy.

Journal Article; Review , 2020

Decalcification of Breast Cancer Bone Metastases With EDTA Does Not Affect ER, PR, and HER2 Results.

van Es SC; Departments of Medical Oncology., van der Vegt B; Pathology., Bensch F; Departments of Medical Oncology., Gerritse S; Department of Medical Oncology, Amsterdam University Medical Center (UMC), VU Medical Center (VUmc), Cancer Center Amsterdam, Amsterdam., van Helden EJ; Department of Medical Oncology, Amsterdam University Medical Center (UMC), VU Medical Center (VUmc), Cancer Center Amsterdam, Amsterdam., Boon E; Department of Medical Oncology, Radboud University Medical Center, Nijmegen., Angus L; Departments of Medical Oncology and Radiology & Nuclear Medicine, Erasmus MC Cancer Institute., Overbosch J; Radiology, University Medical Center Groningen, University of Groningen, Groningen., Menke-van der Houven van Oordt CW; Department of Medical Oncology, Amsterdam University Medical Center (UMC), VU Medical Center (VUmc), Cancer Center Amsterdam, Amsterdam., Verheul HM; Department of Medical Oncology, Amsterdam University Medical Center (UMC), VU Medical Center (VUmc), Cancer Center Amsterdam, Amsterdam., van Herpen CML; Department of Medical Oncology, Radboud University Medical Center, Nijmegen., Jager A; Department of Medical Oncology, Erasmus University Medical Center, Rotterdam, The Netherlands., Oosting SF; Departments of Medical Oncology., de Vries EGE; Departments of Medical Oncology., Schröder CP; Departments of Medical Oncology.

Comparative Study; Journal Article; Research Support, Non-U.S. Gov't , 2019

A novel approach to decalcification in histopathology laboratory: An adaptation from the Hammersmith protocol.

Pradhan P; Currently working in Department of Pathology, Kalinga Institute of Medical Sciences, Bhubaneswar, Odisha, India., Rajesh NG; Department of Pathology, Jawaharlal Institute of Post graduate Medical Education and Research (JIPMER), Puducherry, India., Badhe BA; Department of Pathology, Jawaharlal Institute of Post graduate Medical Education and Research (JIPMER), Puducherry, India., Ilanchezian K; Department of Pathology, Jawaharlal Institute of Post graduate Medical Education and Research (JIPMER), Puducherry, India., Manimehalai D; Department of Pathology, Jawaharlal Institute of Post graduate Medical Education and Research (JIPMER), Puducherry, India., Jyothish A; Department of Pathology, Jawaharlal Institute of Post graduate Medical Education and Research (JIPMER), Puducherry, India.

Journal Article , 2019

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Effects of fixation and demineralization on histomorphology and DNA amplification of canine bone marrow.

Diamantino, Gabriella, Beeler-Marfisi, Janet, Foster, Robert, Sears, William, Defarges, Alice, Vernau, William, Bienzle, Dorothee

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