Search Results - "clustered regularly interspaced short palindromic repeats"

CRISPR GENome and epigenome engineering improves loss-of-function genetic-screening approaches.

Stadager J; University Stuttgart, Department of Molecular Biochemistry, Allmandring 31, 70569 Stuttgart, Germany., Bernardini C; University Stuttgart, Department of Cellular Biochemistry, Allmandring 31, 70569 Stuttgart, Germany., Hartmann L; University Stuttgart, Institute of Biomedical Genetics, Allmandring 31, 70569 Stuttgart, Germany., May H; University Stuttgart, Department of Molecular Biochemistry, Allmandring 31, 70569 Stuttgart, Germany., Wiepcke J; University Stuttgart, Department of Molecular Biochemistry, Allmandring 31, 70569 Stuttgart, Germany., Kuban M; University Stuttgart, Institute of Biomedical Genetics, Allmandring 31, 70569 Stuttgart, Germany., Najafova Z; Robert Bosch Center for Tumor Diseases, Auerbachstraße 112, 70376 Stuttgart, Germany., Johnsen SA; Robert Bosch Center for Tumor Diseases, Auerbachstraße 112, 70376 Stuttgart, Germany., Legewie S; University Stuttgart, Institute of Biomedical Genetics, Allmandring 31, 70569 Stuttgart, Germany., Traube FR; University Stuttgart, Department of Cellular Biochemistry, Allmandring 31, 70569 Stuttgart, Germany., Jude J; TWIST Bioscience, 681 Gateway Boulevard, South San Francisco, CA 94080, USA., Rathert P; University Stuttgart, Department of Molecular Biochemistry, Allmandring 31, 70569 Stuttgart, Germany. Electronic address: philipp.rathert@ibc.uni-stuttgart.de.

Journal Article , 2025

Cas11 augments Cascade functions in type I-E CRISPR system but is redundant for gene silencing and plasmid interference.

Pandey N; Applied Genomics Section, Bio-Science Group, Bhabha Atomic Research Centre, Mumbai, India.; Life Sciences, Mumbai University, Vidya Nagari, Kalina, Santacruz East, Mumbai, India., Misra CS; Applied Genomics Section, Bio-Science Group, Bhabha Atomic Research Centre, Mumbai, India., Rath D; Applied Genomics Section, Bio-Science Group, Bhabha Atomic Research Centre, Mumbai, India.; Homi Bhabha National Institute, Anushaktinagar, Mumbai, India.

Journal Article , 2025

Comparative genomics of Lentilactobacillus buchneri reveals strain-level hyperdiversity and broad-spectrum CRISPR immunity against human and livestock gut phages.

Gumustop I; Department of Molecular Biology and Genetics, Faculty of Arts and Sciences, Bogazici University, Istanbul, Turkiye., Genel I; School of Medicine, Koc University, Istanbul, Turkiye., Kurt IC; Department of Molecular Biology and Genetics, Faculty of Arts and Sciences, Bogazici University, Istanbul, Turkiye., Ortakci F; Department of Food Engineering, Faculty of Chemical and Metallurgical Engineering, Istanbul Technical University, Istanbul, Turkiye.

Journal Article; Comparative Study , 2025

Leveraging CRISPR activation for rapid assessment of gene editing products in human pluripotent stem cells.

Wu Y; The SKI Stem Cell Research Facility, The Center for Stem Cell Biology and Developmental Biology Program, Sloan-Kettering Institute for Cancer Research, 1275 York Avenue, New York, NY 10065, USA., Zhong A; The SKI Stem Cell Research Facility, The Center for Stem Cell Biology and Developmental Biology Program, Sloan-Kettering Institute for Cancer Research, 1275 York Avenue, New York, NY 10065, USA., Evangelisti A; The Center for Stem Cell Biology and Developmental Biology Program, Sloan-Kettering Institute for Cancer Research, 1275 York Avenue, New York, NY 10065, USA., Sidharta M; The SKI Stem Cell Research Facility, The Center for Stem Cell Biology and Developmental Biology Program, Sloan-Kettering Institute for Cancer Research, 1275 York Avenue, New York, NY 10065, USA., Danwei H; The Center for Stem Cell Biology and Developmental Biology Program, Sloan-Kettering Institute for Cancer Research, 1275 York Avenue, New York, NY 10065, USA., Studer L; The Center for Stem Cell Biology and Developmental Biology Program, Sloan-Kettering Institute for Cancer Research, 1275 York Avenue, New York, NY 10065, USA. Electronic address: studerl@mskcc.org., Zhou T; The SKI Stem Cell Research Facility, The Center for Stem Cell Biology and Developmental Biology Program, Sloan-Kettering Institute for Cancer Research, 1275 York Avenue, New York, NY 10065, USA. Electronic address: zhout@mskcc.org.

Journal Article , 2025

CRISPR Digital Sensing: From Micronano-Collaborative Chip to Biomolecular Detection.

Wang X; Ministry of Education Engineering Research Center of Smart Microsensors and Microsystems, School of Electronics and Information, Hangzhou Dianzi University, Hangzhou 310018, China.; Key Laboratory of Micro-Nano Sensing and IoT of Wenzhou, Wenzhou Institute of Hangzhou Dianzi University, Wenzhou 325038, China., Zhong L; Zhejiang Cancer Hospital, Hangzhou Institute of Medicine (HIM), Chinese Academy of Sciences, Hangzhou, Zhejiang 310022, China., Zhang W; Ministry of Education Engineering Research Center of Smart Microsensors and Microsystems, School of Electronics and Information, Hangzhou Dianzi University, Hangzhou 310018, China.; Key Laboratory of Micro-Nano Sensing and IoT of Wenzhou, Wenzhou Institute of Hangzhou Dianzi University, Wenzhou 325038, China., Wu P; Ministry of Education Engineering Research Center of Smart Microsensors and Microsystems, School of Electronics and Information, Hangzhou Dianzi University, Hangzhou 310018, China.; Key Laboratory of Micro-Nano Sensing and IoT of Wenzhou, Wenzhou Institute of Hangzhou Dianzi University, Wenzhou 325038, China., Wang M; Ministry of Education Engineering Research Center of Smart Microsensors and Microsystems, School of Electronics and Information, Hangzhou Dianzi University, Hangzhou 310018, China.; Key Laboratory of Micro-Nano Sensing and IoT of Wenzhou, Wenzhou Institute of Hangzhou Dianzi University, Wenzhou 325038, China., Li D; Ministry of Education Engineering Research Center of Smart Microsensors and Microsystems, School of Electronics and Information, Hangzhou Dianzi University, Hangzhou 310018, China., Dong L; Ministry of Education Engineering Research Center of Smart Microsensors and Microsystems, School of Electronics and Information, Hangzhou Dianzi University, Hangzhou 310018, China., Wang G; Ministry of Education Engineering Research Center of Smart Microsensors and Microsystems, School of Electronics and Information, Hangzhou Dianzi University, Hangzhou 310018, China.

Journal Article; Review , 2025

Self-organization of mouse embryonic stem cells into reproducible pre-gastrulation embryo models via CRISPRa programming.

Lodewijk GA; Department of Biomolecular Engineering, University of California, Santa Cruz, Santa Cruz, CA, USA; Genomics Institute, University of California, Santa Cruz, Santa Cruz, CA, USA; Institute for The Biology of Stem Cells, University of California, Santa Cruz, Santa Cruz, CA, USA., Kozuki S; Department of Biomolecular Engineering, University of California, Santa Cruz, Santa Cruz, CA, USA; Genomics Institute, University of California, Santa Cruz, Santa Cruz, CA, USA; Institute for The Biology of Stem Cells, University of California, Santa Cruz, Santa Cruz, CA, USA., Han CJ; Department of Biomolecular Engineering, University of California, Santa Cruz, Santa Cruz, CA, USA; Genomics Institute, University of California, Santa Cruz, Santa Cruz, CA, USA; Institute for The Biology of Stem Cells, University of California, Santa Cruz, Santa Cruz, CA, USA., Topacio BR; Department of Biomolecular Engineering, University of California, Santa Cruz, Santa Cruz, CA, USA; Genomics Institute, University of California, Santa Cruz, Santa Cruz, CA, USA; Institute for The Biology of Stem Cells, University of California, Santa Cruz, Santa Cruz, CA, USA., Lee S; Department of Biomolecular Engineering, University of California, Santa Cruz, Santa Cruz, CA, USA; Genomics Institute, University of California, Santa Cruz, Santa Cruz, CA, USA; Institute for The Biology of Stem Cells, University of California, Santa Cruz, Santa Cruz, CA, USA., Nixon L; Department of Biomolecular Engineering, University of California, Santa Cruz, Santa Cruz, CA, USA; Genomics Institute, University of California, Santa Cruz, Santa Cruz, CA, USA; Institute for The Biology of Stem Cells, University of California, Santa Cruz, Santa Cruz, CA, USA., Zargari A; Department of Electrical and Computer Engineering, University of California, Santa Cruz, Santa Cruz, CA, USA., Knight G; Neurosetta LLC, Madison, WI, USA; Wisconsin Institute for Discovery, Madison, WI, USA., Ashton R; Neurosetta LLC, Madison, WI, USA; Wisconsin Institute for Discovery, Madison, WI, USA; Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, USA., Qi LS; Department of Bioengineering, Stanford University, Stanford, CA, USA; Sarafan ChEM-H, Stanford University, Stanford, CA, USA; Chan Zuckerberg Biohub, San Francisco, San Francisco, CA, USA., Shariati SA; Department of Biomolecular Engineering, University of California, Santa Cruz, Santa Cruz, CA, USA; Genomics Institute, University of California, Santa Cruz, Santa Cruz, CA, USA; Institute for The Biology of Stem Cells, University of California, Santa Cruz, Santa Cruz, CA, USA. Electronic address: alish@ucsc.edu.

Journal Article , 2025

Dual-Amplification CRISPR Biosensor for Ultrasensitive miRNA Detection: A Powerful Tool for Cancer and Immune Monitoring.

Liu Q; State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Nankai University, Tianjin 300350, P.R. China., Han C; State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Nankai University, Tianjin 300350, P.R. China., Chang X; State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Nankai University, Tianjin 300350, P.R. China., Zhang Z; Nankai International Advanced Research Institute, Shenzhen, Guangdong 518045, P.R. China., Jia C; State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Nankai University, Tianjin 300350, P.R. China.; Nankai International Advanced Research Institute, Shenzhen, Guangdong 518045, P.R. China., Zhang L; State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Nankai University, Tianjin 300350, P.R. China.; Nankai International Advanced Research Institute, Shenzhen, Guangdong 518045, P.R. China.

Journal Article , 2025

Bacteriophages in Pseudomonas aeruginosa evade the CRISPR-Cas I-F system by depletion of PAM sequences.

Ortega-Sanz I; Department of Food Technology, Safety and Health, Faculty of Bioscience Engineering, Ghent University, 9000 Gent, Belgium.; Department of Biotechnology and Food Science, University of Burgos, Burgos, Spain., Rubio A; Andalusian Centre for Developmental Biology (CABD, UPO-CSIC-JA). Faculty of Experimental Sciences (Genetics Area), University Pablo de Olavide, 41013, Seville, Spain., Pérez-Pulido AJ; Andalusian Centre for Developmental Biology (CABD, UPO-CSIC-JA). Faculty of Experimental Sciences (Genetics Area), University Pablo de Olavide, 41013, Seville, Spain.

Journal Article , 2025

Decoding post-transcriptional regulatory networks by RNA-linked CRISPR screening in human cells.

Nugent PJ; Basic Sciences Division and Computational Biology Section of the Public Health Sciences Division, Fred Hutchinson Cancer Center, Seattle, WA, USA.; Molecular and Cellular Biology Graduate Program, University of Washington, Seattle, WA, USA., Park H; Basic Sciences Division and Computational Biology Section of the Public Health Sciences Division, Fred Hutchinson Cancer Center, Seattle, WA, USA., Wladyka CL; Human Biology Division, Fred Hutchinson Cancer Center, Seattle, WA, USA., Yelland JN; Basic Sciences Division and Computational Biology Section of the Public Health Sciences Division, Fred Hutchinson Cancer Center, Seattle, WA, USA., Sinha S; Translational Science and Therapeutics Division, Fred Hutchinson Cancer Center, Seattle, WA, USA., Chen KY; Basic Sciences Division and Computational Biology Section of the Public Health Sciences Division, Fred Hutchinson Cancer Center, Seattle, WA, USA.; Molecular and Cellular Biology Graduate Program, University of Washington, Seattle, WA, USA., Bynum C; Basic Sciences Division and Computational Biology Section of the Public Health Sciences Division, Fred Hutchinson Cancer Center, Seattle, WA, USA.; Department of Biology, Spelman College, Atlanta, GA, USA., Quarterman G; Basic Sciences Division and Computational Biology Section of the Public Health Sciences Division, Fred Hutchinson Cancer Center, Seattle, WA, USA.; Department of Biology, Spelman College, Atlanta, GA, USA., Lee SC; Translational Science and Therapeutics Division, Fred Hutchinson Cancer Center, Seattle, WA, USA., Hsieh AC; Human Biology Division, Fred Hutchinson Cancer Center, Seattle, WA, USA.; Department of Medicine and Department of Genome Sciences, University of Washington, Seattle, WA, USA., Subramaniam AR; Basic Sciences Division and Computational Biology Section of the Public Health Sciences Division, Fred Hutchinson Cancer Center, Seattle, WA, USA. rasi@fredhutch.org.; Department of Biochemistry and Department of Genome Sciences, University of Washington, Seattle, WA, USA. rasi@fredhutch.org.

Journal Article , 2025

Advancing forensic body fluid identification: A comparative analysis of RT-LAMP+CRISPR-Cas12a and established mRNA-based methods.

Martin OL; Forensic Science Programme, University of Auckland, Auckland, New Zealand., Lynch CRH; Forensic Research and Development Team, Institute of Environmental Science and Research Ltd, Auckland, New Zealand., Fleming R; Forensic Research and Development Team, Institute of Environmental Science and Research Ltd, Auckland, New Zealand. Electronic address: rachel.fleming@esr.cri.nz.

Journal Article; Comparative Study , 2025

The L348P point mutation in cardiac myosin binding protein-C alters transient responses to stretch, slows cardiac relaxation, and is embryonic lethal in homozygous CRISPR gene-edited mice.

Sadler RL; Department of Physiology, University of Arizona, Tucson, AZ 85724, USA., Greenman AC; Department of Physiology, University of Arizona, Tucson, AZ 85724, USA., Methawasin M; Department of Cellular and Molecular Medicine, University of Arizona, Tucson, AZ 85724, USA., Fan J; Department of Physiology, University of Arizona, Tucson, AZ 85724, USA., Harris SP; Department of Physiology, University of Arizona, Tucson, AZ 85724, USA. Electronic address: samharris@arizona.edu.

Journal Article , 2025

AcrDB update: Predicted 3D structures of anti-CRISPRs in human gut viromes.

Khatri M; Nebraska Food for Health Center, Department of Food Science and Technology, University of Nebraska-Lincoln, Lincoln, Nebraska, USA., Shanmugam NRS; Nebraska Food for Health Center, Department of Food Science and Technology, University of Nebraska-Lincoln, Lincoln, Nebraska, USA., Zhang X; Nebraska Food for Health Center, Department of Food Science and Technology, University of Nebraska-Lincoln, Lincoln, Nebraska, USA., Patel RSKR; Nebraska Food for Health Center, Department of Food Science and Technology, University of Nebraska-Lincoln, Lincoln, Nebraska, USA., Yin Y; Nebraska Food for Health Center, Department of Food Science and Technology, University of Nebraska-Lincoln, Lincoln, Nebraska, USA.

Journal Article , 2025

Optimization of genome editing by CRISPR ribonucleoprotein for high efficiency of germline transmission of Sox9 in zebrafish.

Yang K; Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, Renmin Hospital of Wuhan University, Wuhan University, Wuhan 430072, China., Cai L; Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, Renmin Hospital of Wuhan University, Wuhan University, Wuhan 430072, China., Zhao Y; Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, Renmin Hospital of Wuhan University, Wuhan University, Wuhan 430072, China., Cheng H; Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, Renmin Hospital of Wuhan University, Wuhan University, Wuhan 430072, China. Electronic address: hhcheng@whu.edu.cn., Zhou R; Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, Renmin Hospital of Wuhan University, Wuhan University, Wuhan 430072, China. Electronic address: rjzhou@whu.edu.cn.

Journal Article , 2025

Development of a CRISPR activation system for targeted gene upregulation in Synechocystis sp. PCC 6803.

Bourgade B; Microbial Chemistry, Department of Chemistry-Ångström Laboratory, Uppsala University, Uppsala, Sweden.; Department of Organismal Biology, Uppsala University, Uppsala, Sweden., Xie H; Microbial Chemistry, Department of Chemistry-Ångström Laboratory, Uppsala University, Uppsala, Sweden.; College of Bioengineering, Sichuan University of Science and Engineering, Yibin, China., Lindblad P; Microbial Chemistry, Department of Chemistry-Ångström Laboratory, Uppsala University, Uppsala, Sweden., Stensjö K; Microbial Chemistry, Department of Chemistry-Ångström Laboratory, Uppsala University, Uppsala, Sweden. karin.stensjo@kemi.uu.se.

Journal Article , 2025

Robust prediction of synthetic gRNA activity and cryptic DNA repair by disentangling cellular CRISPR cleavage outcomes.

Riesenberg S; Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany. stephan_riesenberg@eva.mpg.de., Kanis P; Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany., Karlic R; Bioinformatics Group, Division of Molecular Biology, Department of Biology, University of Zagreb, Zagreb, Croatia., Maricic T; Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany.

Journal Article , 2025

In vivo CRISPR activation screen identifies acyl-CoA-binding protein as a driver of bone metastasis.

Teng H; Department of Experimental Radiation Oncology, University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA., Hang Q; Department of Experimental Radiation Oncology, University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA., Zheng C; Department of Bioinformatics and Computational Biology, University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA., Yan Y; Department of Experimental Radiation Oncology, University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA., Liu S; Department of Experimental Radiation Oncology, University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA., Zhao Y; Department of Experimental Radiation Oncology, University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA., Deng Y; Department of Experimental Radiation Oncology, University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA., Nie L; Department of Experimental Radiation Oncology, University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA., Wu W; Department of Experimental Radiation Oncology, University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA., Sheldon M; Department of Experimental Radiation Oncology, University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA., Yu Z; Department of Chemistry, Rice University, Houston, TX 77005, USA., Shi W; Department of Experimental Radiation Oncology, University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA., Gao J; Department of Orthopedics, Shanghai General Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200080, China.; Tongji University Cancer Center, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai 200080, China., Meng C; Department of Experimental Radiation Oncology, University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA., Martinez C; Department of Experimental Radiation Oncology, University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA., Zhang J; Department of Experimental Radiation Oncology, University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA., Yao F; Department of Experimental Radiation Oncology, University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA., Sun Y; Department of Molecular and Cellular Oncology, University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA., Zhao D; Department of Experimental Radiation Oncology, University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.; University of Texas MD Anderson Cancer Center UTHealth Houston Graduate School of Biomedical Sciences, Houston, TX 77030, USA., Gan B; Department of Experimental Radiation Oncology, University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.; University of Texas MD Anderson Cancer Center UTHealth Houston Graduate School of Biomedical Sciences, Houston, TX 77030, USA., Meng T; Department of Orthopedics, Shanghai General Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200080, China.; Tongji University Cancer Center, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai 200080, China., Ma L; Department of Experimental Radiation Oncology, University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.; University of Texas MD Anderson Cancer Center UTHealth Houston Graduate School of Biomedical Sciences, Houston, TX 77030, USA.

Journal Article , 2025

Array Assembler Provides Greatly Simplified crRNA Array Design for CRISPR Cas12 and Cas13 Variants.

Bryson JW; Biotech Research and Innovation Centre (BRIC), University of Copenhagen, Copenhagen 2200, Denmark.

Journal Article; Research Support, Non-U.S. Gov't , 2025

Deep Learning-Based Classification of CRISPR Loci Using Repeat Sequences.

Liao X; School of Computer Science, Northwestern Polytechnical University, Xi'an, Shanxi 710072, China., Li Y; School of Computer Science, Northwestern Polytechnical University, Xi'an, Shanxi 710072, China., Wu Y; School of Computer Science, Northwestern Polytechnical University, Xi'an, Shanxi 710072, China., Li X; School of Computer Science, Northwestern Polytechnical University, Xi'an, Shanxi 710072, China., Shang X; School of Computer Science, Northwestern Polytechnical University, Xi'an, Shanxi 710072, China.

Journal Article; Research Support, Non-U.S. Gov't , 2025

CRISPR genome editing using a combined positive and negative selection system.

Sharma I; Department of Medicine, University of Florida, GainesvilleFlorida, United States of America., Hall K; Department of Medicine, University of Florida, GainesvilleFlorida, United States of America., Moonah S; Department of Medicine, University of Florida, GainesvilleFlorida, United States of America.; Department of Molecular Genetics and Microbiology, University of Florida, GainesvilleFlorida, United States of America.

Journal Article , 2025

Comprehensive secretome profiling and CRISPR screen identifies SFRP1 as a key inhibitor of epidermal progenitor proliferation.

Cheng B; Department of Dermatology, University of California Irvine, Irvine, CA, USA., Peng SI; Department of Dermatology, University of California Irvine, Irvine, CA, USA., Jia YY; Department of Dermatology, University of California Irvine, Irvine, CA, USA.; Department of Developmental and Cell Biology, University of California Irvine, Irvine, CA, USA., Tong E; Department of Dermatology, University of California Irvine, Irvine, CA, USA., Atwood SX; Department of Dermatology, University of California Irvine, Irvine, CA, USA.; Department of Developmental and Cell Biology, University of California Irvine, Irvine, CA, USA., Sun BK; Department of Dermatology, University of California Irvine, Irvine, CA, USA. sunb8@hs.uci.edu.

Journal Article , 2025