High-throughput solutions in tumor organoids: from culture to drug screening.

Stem cells (Dayton, Ohio) [Stem Cells] 2025 Jan 17; Vol. 43 (1).

Tumor organoids have emerged as an ideal in vitro model for patient-derived tissues, as they recapitulate the characteristics of the source tumor tissue to a certain extent, offering the potential for personalized tumor therapy and demonstrating significant promise in pharmaceutical research and development. However, establishing and applying this model involves multiple labor-intensive and time-consuming experimental steps and lacks standardized protocols and uniform identification criteria. Thus, high-throughput solutions are essential for the widespread adoption of tumor organoid models. This review provides a comprehensive overview of current high-throughput solutions across the entire workflow of tumor organoids, from sampling and culture to drug screening. Furthermore, we explore various technologies that can control and optimize single-cell preparation, organoid culture, and drug screening with the ultimate goal of ensuring the automation and high efficiency of the culture system and identifying more effective tumor therapeutics.
(© The Author(s) 2024. Published by Oxford University Press.)

English

Publisher: Oxford University Press Country of Publication: England NLM ID: 9304532 Publication Model: Print Cited Medium: Internet ISSN: 1549-4918 (Electronic) Linking ISSN: 10665099 NLM ISO Abbreviation: Stem Cells Subsets: MEDLINE

Organoids*/pathology , Organoids*/drug effects , High-Throughput Screening Assays*/methods , Neoplasms*/drug therapy , Neoplasms*/pathology , Cell Culture Techniques*/methods, Humans ; Drug Screening Assays, Antitumor/methods ; Animals

20250425