This technique note proposes two classes of functional and disturbance observers for positive systems with structural and non-structural disturbances, respectively. A positive functional observer is first proposed for positive systems by introducing the estimation of disturbance to the observer. By developing the disturbance observer technique, a positive disturbance observer is designed to supply the estimation of disturbance in the functional observer. Then, a new unknown input observer is constructed for positive systems. A matrix decomposition method is employed to design the observer gains. All conditions are described in terms of linear programming. The corresponding algorithms are addressed for computing the presented conditions. Finally, two examples are provided to verify the effectiveness of the theoretical findings.