LncRNA-miRNA interplay regulate intestinal regeneration in the sea cucumber Apostichopus japonicus

2025

Computational and Structural Biotechnology Journal, Vol 27, Iss , Pp 1383-1393 (2025)

The sea cucumber Apostichopus japonicus, renowned for its remarkable ability to expel and regenerate its internal organs within weeks, serves as a model organism for regeneration research. However, studies on the role of non-coding RNAs, particularly long non-coding RNA (lncRNA), in intestinal regeneration remain limited. In this study, we identified and performed differential expression analysis of lncRNAs in both normal intestines and intestines at 3 days post evisceration (dpe). A total of 2361 lncRNAs were identified, 183 of which were differentially expressed (DE-lncRNAs). The genes targeted by these lncRNAs, either cis- or trans-acting, were involved in oxidative stress, immune response, extracellular matrix remodeling, and energy metabolism during intestinal regeneration. Notably, MSTRG.6200/miR-7847–3p and MSTRG.18440/miR-4220–5p have been confirmed as interacting lncRNA-miRNA pairs. These results suggest that lncRNAs are key regulators of intestinal regeneration in A. japonicus, offering new insights into the underlying mechanisms and potential targets for enhancing regeneration.

article

English

Elsevier, 2025.

Long non-coding RNA (lncRNA), Intestinal regeneration, Echinoderm, Apostichopus japonicus, Biotechnology, TP248.13-248.65