Abstract Background A serious game module called “D-Casting” was developed in the previous study. This study aimed to determine the effectiveness of the D-Casting module. Methods The experiment consisted of two parts: construct validity assessment and skill transfer assessment. Eligible participants, who were students majoring in dental and dental technology, were recruited from the Stomatology College of Chongqing Medical University. The intervention was designed based on the serious games assessment framework. Results The construct validity assessment recruited a total of 145 participants (100% response rate). The results suggested that D-Casting module could effectively distinguish novice and experienced students (86.67[15.832] vs. 88.62 [11.252] vs. 82.24 [15.674] vs. 96.39 [8.419]; P