intercellular interaction, metabolic regulation, microenvironment, skeletal muscle, snRNA‐seq, spatial transcriptome, Diseases of the musculoskeletal system, RC925-935, Human anatomy, QM1-695

ABSTRACT Background Cell–cell crosstalk between myogenic, adipogenic and immune cells in skeletal muscle to regulate energy metabolism and lipid deposition has received considerable attention. The specific mechanisms of interaction between the different cells in skeletal muscle are still unclear. Methods Using integrated analysis of snRNA‐seq and spatial transcriptome, the gene expression profile of longissimus dorsi (LD) muscle was compared between adult Taoyuan black (TB, obese, native Chinese breed) and Duroc (lean) pigs. Results TB pig had more intramuscular fat (IMF) deposition (3.91%, p = 0.0244) and higher slow myofiber proportion (17.13%, p 0.4, p