Lipidomics, Differential lipids, Volatile organic compounds, Giant salamander, Gender, Nutrition. Foods and food supply, TX341-641, Food processing and manufacture, TP368-456

To uncover the relationships between lipid components and flavor volatiles, distinctness in lipid components and odor substances in giant salamander livers of different genders were comparatively characterized through UPLC-Q Exactive-MS lipidomics and gas chromatography-ion migration spectrometry (GC-IMS). A total of 2171 and 974 lipid metabolites were detected in positive and negative ion modes, respectively. Triglycerides (TG) and phosphatidylcholines (PC) are the most abundant types of lipids. TG level in male livers was higher than that in female livers (P 0.05). Additionally, a total of 51 volatile components were detected through GC-IMS. Ketones (42.18 % ∼ 45.44 %) and alcohols (24.19 % ∼ 26.50 %) were the predominant categories, and their relative contents were higher in female livers. Finally, 30 differential lipid metabolites and 12 differential odor substances were screened and could be used as distinguishing labels in giant salamander livers of different genders. Correlation analysis indicated that PS(36:2e), TG(48:13), ZyE(37:6), and ZyE(33:6) correlated positively with 3-methyl butanal, 3-hydroxy-2-butanone, and 2-methyl-1-propanol (P