Purpose: This study aimed to determine the application skills, self-efficacy, and anxiety levels of the mothers whose children receive nebulized inhalation treatment and to examine the relationship between them.Method: This research was a descriptive clinical study. The study was conducted with 300 mothers. Data were evaluated by Pearson correlation and nonparametric tests.Results: Of the mothers, 78.3% were determined to get a moderate score on “The Nebulized Inhalation Application Checklist”. The total Situational Anxiety score of the mothers was calculated to be 37.07±4.95 and the total mean Self-Efficacy score 79.60±15.23. No correlation was found between Self-Efficacy and Situational Anxiety levels of mothers (p gt;0.05).Conclusion: Nurses can contribute to treatment by supporting them to develop training programs to increase self-efficacy and reduce the anxiety of mothers whose children receive inhalation treatment.