Laddar…
Academic Journal
Hierarchical porous ECM scaffolds incorporating GDF-5 fabricated by cryogenic 3D printing to promote articular cartilage regeneration
Jiang Wu, Liwei Fu, Zineng Yan, Yu Yang, Han Yin, Pinxue Li, Xun Yuan, Zhengang Ding, Teng Kang, Zhuang Tian, Zhiyao Liao, Guangzhao Tian, Chao Ning, Yuguo Li, Xiang Sui, Mingxue Chen, Shuyun Liu, Quanyi Guo
Biomaterials Research, Vol 27, Iss 1, Pp 1-18 (2023)
Sparad:
| Titel | Hierarchical porous ECM scaffolds incorporating GDF-5 fabricated by cryogenic 3D printing to promote articular cartilage regeneration |
|---|---|
| Författarna | Jiang Wu, Liwei Fu, Zineng Yan, Yu Yang, Han Yin, Pinxue Li, Xun Yuan, Zhengang Ding, Teng Kang, Zhuang Tian, Zhiyao Liao, Guangzhao Tian, Chao Ning, Yuguo Li, Xiang Sui, Mingxue Chen, Shuyun Liu, Quanyi Guo |
| Utgivningsår |
2023
|
| Källa |
Biomaterials Research, Vol 27, Iss 1, Pp 1-18 (2023)
|
| Beskrivning |
Abstract Background In recent years, there has been significant research progress on in situ articular cartilage (AC) tissue engineering with endogenous stem cells, which uses biological materials or bioactive factors to improve the regeneration microenvironment and recruit more endogenous stem cells from the joint cavity to the defect area to promote cartilage regeneration. Method In this study, we used ECM alone as a bioink in low-temperature deposition manufacturing (LDM) 3D printing and then successfully fabricated a hierarchical porous ECM scaffold incorporating GDF-5. Results Comparative in vitro experiments showed that the 7% ECM scaffolds had the best biocompatibility. After the addition of GDF-5 protein, the ECM scaffolds significantly improved bone marrow mesenchymal stem cell (BMSC) migration and chondrogenic differentiation. Most importantly, the in vivo results showed that the ECM/GDF-5 scaffold significantly enhanced in situ cartilage repair. Conclusion In conclusion, this study reports the construction of a new scaffold based on the concept of in situ regeneration, and we believe that our findings will provide a new treatment strategy for AC defect repair.
|
| Dokumenttyp |
article
|
| Språk |
English
|
| Information om utgivare |
American Association for the Advancement of Science (AAAS), 2023.
|
| Ämnestermer | |
