Ministry of Health of the Russian Federation. Federal State Budgetary Institution «Scientific Centre for Expert Evaluation of Medicinal Products», 2018.

чума, бруцеллез, туляремия, сибирская язва, холера, антиген, иммунная сыворотка крови, реакция непрямой гемагглютинации, иммуноферментный анализ, полимеразная цепная реакция, магноиммуносорбент, пьезокварцевые биосенсорные устройства, plague, brucellosis, tularemia, anthrax, cholera, antigen, immune serum, indirect hemagglutination reaction, immunosorbent assay, polymerase chain reaction, magnesium immunosorbent, piezoelectric quartz crystal biosensor, Biotechnology, TP248.13-248.65, Medicine

The present article describes the scientific and methodological development of biotechnological manufacture of test-system components (diagnostic preparations) for instant diagnosis of plague, brucellosis, tularemia, anthrax, cholera. In this regard, in the first place the effective methods for obtaining complete antigenic complexes used for immunizing animals for the purpose of developing highly potent immune sera have been established. These antisera were used in determining optimum parameters of manufacture on the basis of their diagnosticums. Methodical basis of developing magnetic immunosorbents for selective concentration of infectious agents and their instant diagnosis methods has been mentioned. Moreover, the article describes the development of piezoelectric quartz crystal biosensors to detect plague, brucellosis and tularemia pathogens by gravimetric flow injection analysis, allowing to quickly implement the process of reliable identification of a test pathogen in antigen-antibody complex.