Bifidobacterium animalis, reverse-transcription quantitative polymerase chain reaction, reference genes, RNA-sequencing, fermented skim milk, Dairy processing. Dairy products, SF250.5-275, Dairying, SF221-250

ABSTRACT: Bifidobacterium animalis, one of the predominant bacteria in the intestines of humans and other mammals, is widely added to dairy products. We employed RNA sequencing to analyze gene expression variance on a genome-wide scale and found stable reference genes (RG) in B. animalis. A total of 1,665 genes were identified by analyzing the data from the transcriptome under 4 different conditions, and 13 probable candidate RG with variation coefficient values