Hydrogel patch, Annulus fibrosus defect repair, Chitin, Collagen, Chitin nanocrystal, Medicine (General), R5-920, Biology (General), QH301-705.5

Discectomy is a widely utilized approach for alleviating disc herniation; however, effective repair of postoperative annulus fibrosus (AF) defects remains a significant challenge. This study introduces a hydrogel patch with enhanced mechanical properties for AF repair fabricated using chitin (Ch), collagen (Col), and chitin nanocrystals (ChNCs) through a freeze-thaw cycling technique. The Ch and Col components constitute the matrix of the hydrogel patch, while uniformly dispersed ChNCs act as a nanofiller, markedly improving the mechanical performance (compression strain: 95 %; compression modulus: 0.27 MPa) of the resulting Ch/Col@ChNCs hydrogel patch. The patch demonstrates advantageous properties, including high porosity, superior water absorption, thermal stability, and biodegradability in simulated body fluid. In vitro assessments reveal excellent biocompatibility with AF cells and enhanced collagen deposition. Furthermore, in vivo studies confirm that the patch effectively repairs postoperative disc defects, exhibiting strong integration with surrounding tissues and facilitating the orderly regeneration of fibrous tissue. This innovative hydrogel patch, combining exceptional properties with a straightforward fabrication process, presents a viable strategy for advancing clinical biomaterials for postoperative AF repair.