Publisher: Humana Press Country of Publication: United States NLM ID: 7911509 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1559-0720 (Electronic) Linking ISSN: 01634984 NLM ISO Abbreviation: Biol Trace Elem Res Subsets: MEDLINE

Copper*/blood , Metallothionein*/genetics , Metallothionein*/metabolism , Zinc*/administration & dosage , Zinc*/pharmacology , Zinc*/blood , Coronary Artery Bypass* , Dietary Supplements* , Leukocytes*/metabolism , Leukocytes*/drug effects , Molecular Chaperones*/genetics, Humans ; Male ; Female ; Middle Aged ; Aged ; Ceruloplasmin/metabolism ; Vitamin E/administration & dosage ; Antioxidants/metabolism ; Copper Transport Proteins

Competing Interests: Declarations. Ethics Approval: The trial protocol was approved by the Institutional Review Board of the Ethics Committee of the National Nutrition and Food Technology Research Institute (Tehran, Iran). Consent to Participate: Informed consent was obtained from each patient. Competing interests: The authors declare no competing interests.
The use of zinc supplement may have a negative effect on copper status. The objective of this study was to evaluate the effect of zinc and vitamin E supplementation on copper and zinc biomarkers in patients undergoing coronary artery bypass graft (CABG) surgery. The study was an add-on project to a previously published randomized controlled trial (NCT05402826) on patients undergoing CABG surgery. Patients in the zinc-vitamin E group (n = 40) received oral zinc (120 mg) and vitamin E (1200 international units) 1 day before surgery, followed by 30 mg of zinc and 200 units of vitamin E per day until 21 days after surgery, while those in the control group (n = 38) received placebo. Plasma levels of copper, ceruloplasmin, superoxide dismutase (SOD) activity, as well as leukocyte gene expression of metallothionein 2A (MT2A) and antioxidant protein 1 (ATOX1), were determined 3 and 21 days after surgery. The plasma copper level in the zinc-vitamin E group was significantly lower than the placebo group on the 3rd postoperative day, but no significant between-group differences were observed on day 21. Plasma ceruloplasmin concentration and SOD activity were not different. Relative mRNA expression of leukocyte MT2A was increased at both times (days 3 and 21 in the zinc-vitamin E group compared to placebo, but ATOX1 expression was not affected. Although the plasma copper level was transiently decreased early after surgery in the zinc-vitamin E group, considering the lack of change in other copper biomarkers, it seems that the use of zinc supplements at the dose used in the present study does not have a significant negative effect on the role of intracellular copper.
(© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)