Introduction: work-related stress is a major public and occupational health problem worldwide. Work-related stress is increasing in low-income countries as a result of globalization and changing working conditions. In Ethiopia, little attention has been paid to this issue in the hotel industry and limited research has been done on hotel employees. This study aimed to determine the prevalence of work-related stress and its associated factors among hotel industry employees in Gondar City, north-western Ethiopia. Methods: an institution-based cross-sectional study was conducted among hotel industry employees in Gondar City, from June 9th to July 11th, 2022. Forty-one (41) hotels with 729 research participants were chosen using the cluster sampling technique. Face-to-face interview with a standardized structured questionnaire was used. Bivariate and multivariable logistic regression analyses were calculated to measure the association between work-related stress and independent variables. Adjusted odds ratios (AOR) with 95%CI or p-value < 0.05 was used to explain a statistically significant association. Results: the overall prevalence of work-related stress was 43.3% with 95% CI (39.6, 47.04%). Factors such as non-star hotels [AOR: 2.19, 95%CI (1.48, 3.24)], two-star hotels [AOR: 3.10, 95%CI (1.23, 7.81)], work experience < 2.5 years [AOR: 1.62, 95%CI (1.11, 2.37)], low job control [AOR: 5.17, 95%CI (3.32, 8.07)], high role conflict [AOR: 3.33, 95%CI (2.22, 5.00)], high job ambiguity [AOR: 1.77, 95%CI (1.20, 2.63)] and lack of social support [AOR: 1.54, 95%CI (1.04, 2.29)] were significantly associated with work-related stress. Conclusion: the prevalence of work-related stress was substantially higher among hotel employees. Hotel level/category, inadequate work experience, low job control, high role conflict, high role ambiguity, and a lack of social support were all significantly associated with exposure to work-related stress. Hotel owners and trade unions should work together to design stress management mechanisms and provide job descriptions that clearly describe responsibilities for minimizing identified problems. [ABSTRACT FROM AUTHOR]

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