Klemera-doubal method biological age, Phenotypic age, Homeostatic dysregulation, Allostatic load, Nonalcoholic fatty liver disease, National health and nutrition examination survey, Diseases of the digestive system. Gastroenterology, RC799-869

Abstract Purpose To examine the relationship between biological aging and the prevalence of NAFLD. Method We used the recommended sampling weights to account for the complex survey design of NHANES. The analysis, utilizing data from 2005 to 2016, aimed to investigate the impact of biological aging on NAFLD prevalence using various statistical methods. A restricted cubic spline (RCS) model was applied to explore the dose-response relationship, while logistic regression examined linear associations. The robustness of the association across different subgroups was also tested. Result The study included 2786 participants. We found significant associations between NAFLD and the following biological aging metrics: AL score (OR (95%CI) = 1.1932 (1.0597 ~ 1.3435), P = 0.0035), HD (OR (95%CI) = 1.2092 (1.0565 ~ 1.3839), P = 0.0058), and PA (OR (95%CI) = 1.7564 (1.1949 ~ 2.5818), P = 0.0042). All biological aging metrics were identified as independent predictors. PA was most associated with the prevalence of NAFLD. The associations persisted across most subgroups. Conclusion The prevalence of NAFLD was associated with biological aging, emphasizing the importance of addressing potential health risks related to aging.