This study extracted Bambusa blumeana fiber using alkaline boiling and then applied double-roll pressing in order to develop it, explore the applications of its various parts, and improve its utilization potential. The results showed that the outer bamboo fibers are finer and straighter than the inner and middle fibers, and the fracture mode of the bamboo fibers is brittle. From the inside to the outside, the tensile strength of the fiber bundle gradually increases from the top to the bottom. Moreover, the tensile strength of the outside bamboo fiber is twice that of the inside, reaching a maximum of 982 MPa. The surface of the interior bamboo fiber is relatively smooth and can be used in textile and decorative fields. Compared with internal and central bamboo fiber, the outer fiber has higher thermal stability and higher crystallinity, which makes it more advantageous in the process of strengthening composite materials. Studying the structures of fibers from different parts of Bambusa blumeana can provide substantial scientific support for the differential applications of bamboo fibers.