Abstract Background The association between Kyoto Encyclopedia of Genes and Genomes (KEGG) metabolic pathways and immunologic non-response among people living with HIV (PLHIV) on antiretroviral therapy (ART) is not well documented. This study aimed to characterize KEGG metabolic pathways among HIV-infected men who have sex with men (MSM) with different immunologic responses. Methods We recruited HIV-uninfected MSM (healthy controls, HC) and HIV-infected MSM on ART > 24 months in Guangzhou, June-October 2021. HIV-infected MSM with HIV viral load