In this study, goat's milk was fermented by Saccharomyces cerevisiae mixed with six strains of Lactobacillus, respectively, to produce goat's milk wine, and goat’s milk fermented with Saccharomyces cerevisiae was used as the control. Changes in the physicochemical properties, antioxidant activity and volatile compounds of goat's milk during fermentation process were studied. The results showed that the addition of lactic acid bacteria accelerated the decrease in total sugar content of goat's milk wine at the early stage of fermentation compared to the control, and the total sugar content of goat's milk wine fermented with Lactobacillus fermentum decreased significantly from 469.46 g/L to 210.41 g/L after 1 d of fermentation. The addition of lactic acid bacteria resulted in a significant increase in the scavenging capacity of ABTS+ radical and hydroxyl radical in fermented goat's milk wine (P