Response surface methodology, optimization of extraction process, antioxidant activity, Acer mono, bark, Plant culture, SB1-1110, Botany, QK1-989

Response surface methodology (RSM) was used for finding the optimum extraction condition of Acer mono bark. Twenty experimental conditions were set based on three key variables such as temperature, time and pressure by signalling reaction variables with 5 levels of - 2, - 1, 0, 1, 2 in accordance with central composite design for proceeding extraction and antioxidant tests. The optimized condition for the highest extraction yields was 13.10% at 83.48°C, 54.36 MPa for 13.08 minutes. For DPPH radical scavenging ability, an optimal condition was 92.89% at 88.50°C, 49.69 MPa for 15.08 minutes, and for SOD-like activity 40.69% at 85.21°C, 53.28 MPa for 15.83 minutes. The optimized condition for total polyphenol content was 4.23 mg/g at 81.51°C, 52.92 MPa for 14.79 minutes. The most optimized extraction condition was determined to be at 85°C, 52 MPa for 14 minutes for considering both extraction yield and its biological activities of this plant.