Cervical lymph node, Tuberculosis, Metagenomic next-generation sequencing, Metagenomic third-generation sequencing, Ultrasound-guided puncture biopsy, Microbiology, QR1-502

Objectives: The aim of this study was to assess the clinical utility of metagenomic next-generation sequencing (mNGS) and metagenomic third-generation sequencing (mTGS) in diagnosing cervical lymph node tuberculosis through analysis of lymph node tissue. Methods: This study recruited 101 patients with suspected cervical lymph node tuberculosis and took samples under ultrasound guidance. Parallel culture, X-pert, mNGS and mTGS workflows to each sample were performed. Taking clinical diagnosis as the gold standard, We comparatively compared diagnosis performance of the four methods. Results: Clinically, 76 cases were diagnosed as lymph node tuberculosis and 20 cases were non-lymph node tuberculosis. Compared with clinical diagnosis, the sensitivities of mNGS and mTGS were 89.47 % and 82.89 %, respectively, significantly higher than those of culture and X-pert which were 43.28 % and 68.42 %, respectively (P