Wound is known as one of the serious health issue. Boswellia serrata (BS) contains a wide range of phytochemicals with different pharmacological properties. The study aimed to assess the wound healing potential of Boswellia Serrata Extract (BSE) against an experimental model of excision wound. A total of 45 rats in five equal groups were used in this investigation. The wound was created in the cervical dorsal region of each rat as a two-symmetrical circle pattern. All groups were being treated as follow: control group with ointment base, β-Sitosterol group with 0.25% w/w β-Sitosterol-containing ointment, three BSE ointment groups; treated with BSE ointment containing 5%, 10%, and 20% boswellic acid (BA) respectively. The wounds were followed up for 15 days. On days 5, 10, and 15, three rats from each group were sacrificed, the wounds and scars were sent for histopathological study. The ointments containing 5% and 10% BA exerted a significantly increased wound contraction rate, lesser surface area and shorter re-epithelialization period in the macroscopical analysis in compare to the control group and the β-Sitosterol group. However, the microscopical results exhibited a significant healing progress in a concentration dependent-manner. This was reflected by formation of clear dermal granulation tissue, collagen deposition and angiogenesis in quantitative histological evaluation. In conclusion, this study provided a macroscopical and microscopical evidences for a potential wound healing effect of the topical application of BSE in concentration dependent manner. Although the highest BA concentration in macroscopical analysis exhibited lesser efficacy.