Abstract Food safety knowledge and practices are essential to prevent contamination, reduce health risks, and ensure the overall quality and safety of food products, particularly in perishable goods like fish. Therefore, it is crucial to assess the food safety knowledge and practices among fish sellers in various fish markets to see the scenario of safety and hygiene conditions in these markets. This study examined the level of food safety knowledge and its implementation among fish sellers in the Cumilla district of Bangladesh. Data were collected through a structured questionnaire by direct interviews with 100 respondents, comprising 75 retailers and 25 wholesalers from fish markets across eight upazilas. Knowledge regarding foodborne diseases was minimal, as 44% of retailers and wholesalers disagreed that such diseases affect everyone. Regarding food safety knowledge, two-thirds of respondents correctly answered questions about cross-contamination, while only half provided accurate responses related to foodborne diseases and personal hygiene. The composite score of food safety knowledge, attitudes, and practices indicated significant differences (p