Background: Vine tea, derived from selenium-rich regions of China, has been shown through systematic pharmacological approaches to potential
Background: Vine tea, derived from selenium-rich regions of China, has been shown through systematic pharmacological approaches to potentially exhibit significantly superior anti-inflammatory effects in dextran sodium sulfate-induced colitis. However, the mechanism of action is still unclear.Materials and methods: This study analyzed the total flavonoid fraction of Vine tea (VTF) using untargeted metabolomics with UHPLC-OE-MS mass spectrometry. The chemical composition of the VTF samples was characterized through a comprehensive review of literature reports and relevant databases for network pharmacology. We constructed a visualization network linking VTF, compounds, pathways, and ulcerative colitis using GO and KEGG analyses. To evaluate the therapeutic effects of VTF on UC, we established a mouse model of ulcerative colitis induced by 3 % dextran sodium sulfate and assessed the effects of VTF treatment. Additionally, a cellular model was developed, and the Cell Counting Kit-8 assay was used to determine the optimal dosing concentration of VTF in neutrophils. Laser confocal microscopy was employed to analyze the co-localization of P62-LC3 and the expression of Citronelated Histone H3 following VTF intervention.Results: This study identified the five main components of total flavonoids in Vine tea using UHPLC-OE-MS non-targeted metabolomics. We explored the potential mechanisms of VTF intervention in ulcerative colitis using network pharmacology. This analysis identified 112 linker genes. The GO and KEGG enrichment analyses suggested that the inhibitory effect of VTF on intestinal inflammation might be related to the PI3K-AKT-mTOR signaling pathway. In vivo experimental validation revealed that VTF significantly increased colon length and decreased the Disease Activity Index score (P
