atractylodes macrocephala koidz (pamk) polysaccharide, mice, kidney injury, cyclophosphamide, transcriptome sequencing, Food processing and manufacture, TP368-456

This study aimed to investigate the effects of the polysaccharide of Atractylodes macrocephala Koidz (PAMK) on cyclophosphamide (CTX)-induced renal injury in mice and its potential underlying mechanisms. One hundred male C57BL/6 mice, aged 42~43 days, were randomly divided into four groups, with five repetitions in each group and five mice in each repetition. The PAMK group and PAMK+CTX group were orally administered 200 mg/kg PAMK once daily, while the control group and CTX group were given an equivalent amount of saline. From days 25~27 of the experiment, the CTX group and PAMK+CTX group were intraperitoneally injected with 100 mg/kg CTX once daily, while the control group and PAMK group were injected with an equivalent amount of saline. On day 35 of the experiment, the kidneys were collected for histological observation, oxidative stress detection, and transcriptome sequencing. The results showed that compared with the CTX group, the renal injury in the PAMK+CTX group was alleviated. The content of malondialdehyde (MDA) in the kidneys was significantly decreased (P