Background: Ethiopia is one of the countries with a significant shortage of medical blood. Patient caregivers can serve as an important pool of potential blood donors for many reasons. However, limited data are available on the willingness of patient caregivers to donate blood in Ethiopia. Therefore, this study assessed patient caregivers’ willingness to donate blood and associated factors in northwest Ethiopia. Methods: An institution-based cross-sectional study was conducted among 342 patient caregivers at the University of Gondar Comprehensive Specialized Hospital. A systematic random sampling technique was used to select the study participants. Data were collected using a pretested and interviewer-administered questionnaire. Data were entered into EPI data version 4.6 and exported to SPSS version 25 for analysis. The level of significance in the last model was declared based on a p-value of