Objective: To investigate the effect of fruitflow, a water-soluble tomato extract, on platelet function in participants. Methods: 190 participants were completed this clinical trial. The participants were randomly divided into four groups: placebo, fruitflow, aspirin, and fruitflow + aspirin. These groups received the respective supplements for 7 days. Results: Fruitflow intervention for 7 days decreased platelet aggregation ratio by 7.7% and 10.2% in adenosine diphosphate (ADP)- and collagen-stimulated platelets, respectively. Aspirin decreased platelet aggregation ratio by 9.4% and 39% in ADP- and collagen-stimulated platelets, respectively. The combination of fruitflow and aspirin did not exert synergistic inhibitory effect on platelet aggregation. Fruitflow also significantly reduced plasma thromboxane B2 (TXB2), 6-keto-prostaglandin F1α (6-keto-PGF1α), and platelet factor 4 (PF4) levels. Conclusions: Fruitflow intervention decreased platelet aggregation rate, this suggests that fruitflow is a functional food for reducing platelet activity.