Abstract The Golgi apparatus (GA) plays a main role in the protein secretory pathway. Previously, we described a greater GA vesicle density in patients with dilated cardiomyopathy (DCM), as well as an increase in natriuretic peptide (NP) levels inside these vesicles. GA fragmentation could increase the rate of protein transport; for this reason, we aimed to delve deeper into these GA vesicle density alterations by studying the expression of genes related to GA architecture in DCM and its relationship with NP levels. We performed RNA-seq analysis on explanted hearts from DCM patients (n = 13) and control (CNT) individuals (n = 10). We detected alterations in molecules related to the structure and positioning of GA, highlighting the decrease in GM130 levels and increase in the p-GM130/GM130 ratio (p