chemiluminescence magnetic enzyme immunoassay, lead, rapid quantification, grain, Food processing and manufacture, TP368-456, Nutrition. Foods and food supply, TX341-641

An automatic chemiluminescence magnetic enzyme immunoassay based on the high specificity of antigen and antibody was developed for rapid and quantitative determination of lead in grain. By optimizing the pre-treatment conditions and the reaction conditions of chemiluminescence magnetic enzyme immunoassay, the automatic determination of lead in rice, wheat and corn samples was realized. The detection range was 0.05~0.71 mg/kg, and the limit of detection was 0.02 mg/kg in rice and corn and 0.01 mg/kg in wheat. The recovery range was 89.2%~113.6%, and the relative standard deviations were all less than 10%. Based on the chemiluminescence immune platform, the developed method can realize eight samples simultaneous auto-detection within 30 min. Meanwhile, the automatic detection method can avoid human errors, and greatly improve the accuracy and efficiency, showing a good application prospect in the rapid quantitative detection of lead in grain.