Abstract Background Occupational voice users frequently face the challenge of vocal fatigue, impacting their overall well-being and job performance. This study aimed to investigate the knowledge, attitude, and practice (KAP) toward vocal fatigue among occupational voice users. Methods This web-based cross-sectional study was conducted among occupational voice users between July and October 2023 in Shenzhen People’s Hospital, utilizing a self-administered questionnaire to examine the KAP of vocal fatigue. A total of 920 valid questionnaires were collected with 248 males and 672 females, with 34.35% aged between 21 and 30 years. Results The mean (standard deviation) scores were 15.1 (5.3) for knowledge (range: 0–24), 31.6 (3.6) for attitude (range: 8–40), and 45.0 (7.5) for practice (range: 13–65). A graduate degree or higher was positively associated with knowledge (odds ratio [OR] = 1.596, P = 0.007). Negative associations with knowledge included being a salesperson (OR = 0.342, P