ObjectivesThe soft tissue of the palate is the most frequently used donor side for connective tissue grafts. Various techniques have been described to harvest the connective tissue in anterior and posterior regions of the palate (Hürzeler 1999, Jung 2008, Zucchelli 2010). The present study assessed the histological composition of the soft tissue of the palate in the premolar and tuberosity region and compared the histological composition of connective tissue grafts harvested by two different techniques.MethodsTissue samples of the palatal soft tissue of 10 fresh human dentate cadaver heads were harvested in the premolar and tuberosity region. After histological processing, a histomorphometric analysis on the ratio between epithelium, connective tissue, fatty/glandular tissue, and vascular tissue was performed. Height and composition of the total palatal tissue and of digitally marked grafts (two different harvesting techniques: split-flap- and de-epithelialization-technique) were assessed in both regions (premolar and tuberosity) in an area close and more distant from the teeth (Figure 1).ResultsThe height measurements of the palatal soft tissue ranged from 2.4 to 6.9mm. The main parameters (ratio of connective tissue and fatty/glandular tissue) presented no significant difference between the various regions (close and distant areas in the premolar and tuberosity region; p>0.145; Table 1). But significant differences were detected for the histological compositions of the connective tissue grafts (Table 2); the tissue gained by de-epithelialization in the tuberosity region contained a significantly higher amount of connective tissue, than the tissue gained by split-flap-technique in the premolar region (73.3 vs. 56.5%; p=0.041; Figure 2). Altogether, both, height measurements and composition of the palatal tissue, presented a high inter-individual variability (e.g., percentage of fatty/glandular tissue ranged from 0.04 to 73.8%; Figure 3). Comparison between genders revealed significantly higher values of connective tissue in the premolar region of males (p=0.045); all other parameters presented no relevant gender differences (p>0.077).ConclusionsThus far, the connective tissue harvested in the tuberosity region, which is most often done by de-epithelialization to prevent injury to the greater palatine artery (Figure 1), was clinically described to be denser and more firm compared to the tissue gained in the premolar region (most often by split-flap-technique) (Zuhr 2014). The present study is, to the best of our knowledge, the first to prove this clinical assumption with a histomorphometric analysis. Topic of future clinical trials will be to assess, whether the outcome of root coverage procedures is influenced by the histological composition of the graft (more or less fatty).